Monte Carlo model to describe depth selective fluorescence spectra of epithelial tissue: applications for diagnosis of oral precancer.
Monte Carlo model to describe depth selective fluorescence spectra of epithelial tissue: applications for diagnosis of oral precancer.
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蒙特卡罗模型描述上皮组织的深度选择性荧光光谱：在口腔癌前病变诊断中的应用。
DOI：
10.1117/1.3006066
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发表时间：
2008
影响因子：
3.5
通讯作者：
Richards-Kortum,Rebecca
中科院分区：
文献类型：
--
作者：
Pavlova,Ina;Weber,CrystalRedden;Schwarz,RichardA;Williams,Michelle;El-Naggar,Adel;Gillenwater,Ann;Richards-Kortum,Rebecca
我们提出了一个蒙特卡罗模型来预测荧光光谱的口腔粘膜获得的深度选择性光纤探针作为组织的光学特性的函数。模型的灵敏度分析确定如何与肿瘤的发展影响光谱的强度和形状的光学参数的变化，并阐明了正常和癌前口腔部位的光谱差异的生物学基础。预测表明，光谱的口腔粘膜收集的深度选择性探针的上皮光学特性的变化，并在较小程度上，由浅表基质参数的变化，但不是由更深的基质的光学特性的变化。深度选择性探针提供增强的上皮荧光检测，90%的检测信号来自上皮和浅表基质。预测的深度选择性光谱与测量的平均光谱从正常和发育不良的口腔网站是在良好的协议。与发育异常进展相关的参数变化导致荧光强度降低和光谱向更长发射波长的偏移。荧光减少是由于检测到的基质光子减少，而光谱形状的变化是由于上皮中检测到的光子比例增加。
We present a Monte Carlo model to predict fluorescence spectra of the oral mucosa obtained with a depth-selective fiber optic probe as a function of tissue optical properties. A model sensitivity analysis determines how variations in optical parameters associated with neoplastic development influence the intensity and shape of spectra, and elucidates the biological basis for differences in spectra from normal and premalignant oral sites. Predictions indicate that spectra of oral mucosa collected with a depth-selective probe are affected by variations in epithelial optical properties, and to a lesser extent, by changes in superficial stromal parameters, but not by changes in the optical properties of deeper stroma. The depth selective probe offers enhanced detection of epithelial fluorescence, with 90% of the detected signal originating from the epithelium and superficial stroma. Predicted depth-selective spectra are in good agreement with measured average spectra from normal and dysplastic oral sites. Changes in parameters associated with dysplastic progression lead to a decreased fluorescence intensity and a shift of the spectra to longer emission wavelengths. Decreased fluorescence is due to a drop in detected stromal photons, whereas the shift of spectral shape is attributed to an increased fraction of detected photons arising in the epithelium.