Cloning and structure analysis of an NBS-LRR disease-resistant gene from Setaria italica Beauv
Cloning and structure analysis of an NBS-LRR disease-resistant gene from Setaria italica Beauv
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谷子NBS-LRR抗病基因的克隆及结构分析
DOI：
10.1007/s11703-009-0035-9
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
Jingao Dong
中科院分区：
文献类型：
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作者：
Q. Weng;Zhiyong Li;J. Xing;Z. Dong;Jingao Dong
以禾谷（Setaria italicaBeauv）为材料，对NBS-LRR抗病基因保守序列进行简并PCR扩增。品种十粒香对谷子锈菌（Uromyces setariae-italicae）具有抗性。利用Genomic Walking技术获得了一段长度为2673 bp的序列。核苷酸序列包含一个开放阅读框，编码891个氨基酸残基，计算分子量为101.44 kDa。RUS 1（Resistance against Uromyces setariae-italicae，GenBank No.FJ467296）。该基因含有一个NB-ARC结构域和三个保守基序P-loop、激酶2和激酶3，具有NBS-LRR类植物抗病基因的特征。系统发育分析表明，该基因与RPM 1相似，可能属于LZ-NBS-LRR类型的抗病基因。Southern杂交结果表明，谷子基因组中至少存在3个RUS 1拷贝。
Degenerate PCR primers targeting conserved motifs of most NBS-LRR disease-resistant genes in plants were tested inSetaria italicaBeauv. cultivar Shilixiang, which is resistant toUromyces setariae-italicae. A sequence with a length of 2673 bp has been obtained by using Genomic Walking technology. The nucleotide sequence contained an open reading frame that encoded 891 amino acid residues with a calculated molecular mass of 101.44 kDa. It was namedRUS1(Resistance againstUromyces setariae-italicae, GenBank No. FJ467296). It contained an NB-ARC domain and three conserved motifs P-loop, kinase 2, and kinase 3, which had the characteristics of NBS-LRR type resistant gene of plant. Phylogenetic analysis indicated that it was similar toRPM1and might belong to LZ-NBS-LRR type disease resistance gene. Southern blotting result displayed that there were at least three copies ofRUS1in the foxtail millet genome.