Molecular phylogenetic relationship of Tetraodon pufferfish based on mitochondrial DNA analysis
Molecular phylogenetic relationship of Tetraodon pufferfish based on mitochondrial DNA analysis
复制标题
DOI：
10.1007/s12562-013-0598-5
复制
发表时间：
2013-03-01
影响因子：
1.9
通讯作者：
Watabe, Shugo
中科院分区：
文献类型：
--
作者：
Igarashi, Yoji;Doi, Hiroyuki;Watabe, Shugo
属于四齿鱼属的河豚鱼是四齿鱼科中最大的群体之一。这一组包括超过20种分布在淡水，以及咸淡水和沿海水域，非洲和东南亚。然而，它们的系统发育和进化关系仍然不明确。本研究对从上述不同地区采集的17种四齿兽进行了16S rRNA和细胞色素b的线粒体基因测序。以50种四齿兽（含7种四齿兽）为主干的树为基础，对两种四齿兽基因全序列进行超矩阵分析。该系统发育树将四齿兽物种分为三组，与它们的栖息地有很好的相关性，如亚洲淡水、亚洲咸淡水和非洲淡水。亚洲淡水物种T. cochinchinensis的耐盐性明显低于亚洲半咸淡水物种T. nigroviridis，表明亚洲淡水类群中四齿龙物种的形成是由渗透调节相关的分子进化引起的。
Pufferfishes belonging to the genus Tetraodon form one of the largest groups in the family Tetraodontidae. This group consists of more than 20 species distributed across freshwater, as well as brackish and coastal waters, of Africa and Southeast Asia. However, their phylogenetic and evolutionary relationships are still ambiguous. In the present study, mitochondrial genes encoding 16S rRNA and cytochrome b were sequenced for 17 Tetraodon species collected from various areas mentioned above. Supermatrix analysis based on the complete sequences of the two genes from Tetraodon species was performed using a tree with 50 tetraodontid species (including 7 Tetraodon species) as a backbone. The obtained phylogenetic tree classified Tetraodon species into three groups, correlating well with their habitats, such as Asian freshwater, Asian brackish water, and African freshwater. We also showed that salinity tolerance of Asian freshwater species T. cochinchinensis was apparently lower than that of Asian brackish water species T. nigroviridis, suggesting that the speciation of Tetraodon species in the Asian freshwater group was caused by the molecular evolution associated with osmotic regulation.