Influence of irradiation or thymidine (TdR) on the pattern of 3H-TdR incorporation at each cell position in the crypts of the small intestine of the mouse.
Influence of irradiation or thymidine (TdR) on the pattern of 3H-TdR incorporation at each cell position in the crypts of the small intestine of the mouse.
复制标题
辐射或胸苷 (TdR) 对小鼠小肠隐窝中每个细胞位置的 3H-TdR 掺入模式的影响。
DOI：
--
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
C. Potten
中科院分区：
文献类型：
--
作者：
S. Chwalinski;C. Potten
使用放射自显影方法注意到，小肠隐窝底部的S期细胞是外源性注射胸苷最有效的清除者。3 H-TdR（DNA合成的补救途径）被隐窝基部（干细胞区）的细胞掺入的效率是隐窝顶部（成熟增殖细胞）的S期细胞的两倍。在3 H-UdR掺入（DNA合成的从头途径）方面没有这种位置依赖性差异。放射（0.75-5.0戈伊~（137）Cs γ射线）能迅速抑制~ 3 H-TdR掺入，且呈位置依赖性。地穴底部的细胞受到的影响最大。在~ 3 H-TdR前注射冷胸腺嘧啶核苷改变了~ 3 H-TdR沿着侧隐窝的掺入模式，其方式与辐射非常相似，且颗粒数主要在较低位置的细胞中减少。内源性胸苷的区域梯度的存在的可能性（再利用从肠道来源），和辐射的影响，胸苷掺入的梯度导致全身暴露的直接和abscopal效应，进行了讨论。
Using autoradiographic methods it was noted that S phase cells at the bottom of the crypts in the small intestine were the most efficient scavengers of exogenous injected thymidine. The efficiency of the incorporation of 3H-TdR (salvage pathway of DNA synthesis) by cells at the crypt base (stem cell zone) was twice as high as for the S phase cells at the top of the crypt (maturing proliferative cells). There were no such position-dependent differences in incorporation of 3H-UdR (de novo pathway of DNA synthesis). Radiation (0.75-5.0 Gy 137Cs gamma-rays) inhibited the incorporation of 3H-TdR very rapidly and this was also cell-position dependent. The cells at the bottom of the crypt were the most affected. The injection of cold thymidine before 3H-TdR changed the pattern of the incorporation of 3H-TdR along the side of the crypt in a very similar way to radiation, and the grain number was decreased predominantly in the cells at lower positions. The possibility of the existence of a regional gradient of endogenous thymidine (reutilization from intestinal sources), and the influence of irradiation on the gradient of thymidine incorporation resulting from direct and abscopal effects of whole body exposure, are discussed.
培养的 IEC-17 正常大鼠肠上皮细胞对 X 辐射的反应。
影响因子：
3.4
作者：
Sierra,E;Sahu,SK;Chiang,YW;StClair,WH;Osborne,JW
通讯作者：
Osborne,JW