A short peptide derived from the gN helix domain of FGF8b suppresses the growth of human prostate cancer cells.
A short peptide derived from the gN helix domain of FGF8b suppresses the growth of human prostate cancer cells.
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DOI：
10.1016/j.canlet.2013.06.001
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发表时间：
2013-10
期刊：
影响因子：
9.7
通讯作者：
Tao Li;Wu Luo;D. He;Rui-xue Wang;Yadong Huang;Xiangfeng Zeng;Wenhui Wang;Xilei Chen;Susu Gao;Yonglin Yu;Xiaokun Li;Xiao-ping Wu
中科院分区：
文献类型：
--
作者：
Tao Li;Wu Luo;D. He;Rui-xue Wang;Yadong Huang;Xiangfeng Zeng;Wenhui Wang;Xilei Chen;Susu Gao;Yonglin Yu;Xiaokun Li;Xiao-ping Wu
Previous studies have demonstrated that fibroblast growth factor 8b (FGF8b) is up-regulated in a large proportion of prostate cancer patients and that it plays a key role in prostate carcinogenesis. In this study, we designed and synthesized a gN helix domain derived short peptide (termed 8b-13) based on the analysis of the FGF8b–FGFR structure. The synthetic peptides inhibited the proliferation of prostate cancer cell lines, including PC-3 and DU-145 cells. Further investigations indicated that 8b-13 arrested the cell cycle at the G0/G1 phase, reduced the activation of the Erk1/2, P38, and Akt cascades, and down-regulated the expression of G1/S-specific cyclinD1. The suppression of DNA synthesis and the G1 to S phase transition due to the expression of proteins related to proliferation and cell cycle progression may contribute to the inhibitory effect of 8b-13 peptides on cellular proliferation. Our results not only suggest that 8b-13 exerts an antitumor effect in prostate cancer but also confirm the essential role of the gN helix domain in mediating the activity of FGF8b.
