Effect of subcultivation of human bone marrow mesenchymal stem on their capacities for chondrogenesis, supporting hematopoiesis, and telomea length
Effect of subcultivation of human bone marrow mesenchymal stem on their capacities for chondrogenesis, supporting hematopoiesis, and telomea length
复制标题
DOI：
10.1007/s10616-005-3752-9
复制
发表时间：
2005-01-01
期刊：
影响因子：
2.2
通讯作者：
Yoshida, T
中科院分区：
文献类型：
--
作者：
Nakahara, M;Takagi, M;Yoshida, T
研究了人骨髓间充质干细胞传代培养对其软骨形成能力、支持造血能力和端粒长度的影响。从人骨髓抽提物中分离骨髓间充质干细胞，在37℃、5%CO2气氛下，使用含10%胎牛血清的DMEM培养液进行多次传代，直至20个群体倍增水平(PDL)。随着PDL的增加，CD45(-)CD10(+)细胞所占比例略有增加。颗粒细胞培养后，Aggrecan mRNA表达和藏红花O染色证实，这些细胞的成软骨能力无明显变化。不同PDL的细胞间未观察到脐血细胞支持造血细胞能力的变化。另一方面，原代骨髓间充质干细胞的端粒长度随着供者年龄的增加而增加，但随着PDL的增加，端粒长度明显减少。
Effects of subcultivation of human bone marrow mesenchymal stem cells on their capacities for chondrogenesis and supporting hematopoiesis, and telomea length were investigated. Mesenchymal stem cells were isolated from human bone marrow aspirates and subcultivated several times at 37 degrees C under a 5% CO2 atmosphere employing DMEM medium containing 10% FCS up to the 20th population doubling level (PDL). The ratio of CD45(-) CD10(+) cells among these cells slightly increased as PDL increased. However, there was no marked change in the chondrogenic capacity of these cells, which was confirmed by expression assay of aggrecan mRNA and Safranin O staining after pellet cell cultivation. The change in capacity to support hematopoiesis of cord blood cells was not observed among cells with various PDLs. On the other hand, telomere length markedly decreased as PDL increased at a higher rate than that at which telomere length of primary mesenchymal stem cells decreased as the age of donor increased.