Suprabasal 40 kd keratin (K19) expression as an immunohistologic marker of premalignancy in oral epithelium.
Suprabasal 40 kd keratin (K19) expression as an immunohistologic marker of premalignancy in oral epithelium.
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基底上 40 kd 角蛋白 (K19) 表达作为口腔上皮癌前病变的免疫组织学标志物。
DOI：
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发表时间：
1989
影响因子：
6
通讯作者：
James G. Rheinwaldtt
中科院分区：
文献类型：
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作者：
Kristina Lindberg;James G. Rheinwaldtt
作者研究了角蛋白19在正常口腔粘膜和口腔病变中的表达，这些病变表现出一系列被认为是鳞状细胞癌之前的组织病理学变化。福尔马林固定，石蜡包埋的切片用链霉蛋白酶预处理，并用K19特异性抗体通过抗生物素蛋白-生物素免疫过氧化物酶法染色。在非角化粘膜，无论是正常或良性增生，K19是可检测的基底细胞层。在角化粘膜中，无论是正常的还是良性增生的，都没有检测到K19。任何口腔部位的所有病变，经苏木精和伊红染色切片诊断为中度至重度异型增生或原位癌，无论是否角化过度，基底和基底上细胞层中的K19均强烈染色。K19阳性的细胞层的数量与上皮中持续存在异型增生的水平相关。基底上K19染色倾向于发生在终末分化蛋白外皮蛋白表达延迟或缺失的上皮区域。因此，K19的表达可能与干细胞特征的保留或以其他方式不致力于终末鳞状分化的状态有关。基底上K19染色与口腔上皮癌前病变明显相关，因此有望成为口腔组织病理学诊断的有用工具。
The authors have studied the expression of keratin 19 in normal oral mucosa and in oral lesions exhibiting a range of histopathologic changes that are thought to precede squamous cell carcinoma. Formalin-fixed, paraffin-embedded sections were pretreated with pronase and stained with a K19-specific antibody by the avidin-biotin immunoperoxidase method. In nonkeratinized mucosa, whether normal or benign hyperplastic, K19 was detectable in the basal cell layer. In keratinized mucosa, whether normal or benign hyperplastic, there was no detectable K19. All lesions from any oral site that exhibited atypia diagnosed from hematoxylin and eosin stained sections as moderate-to-severe dysplasia or carcinoma in situ, whether hyperkeratotic or not, stained strongly for K19 in the basal and suprabasal cell layers. The number of cell layers that were K19-positive correlated with the level in the epithelium to which dysplasia persisted. Suprabasal K19 staining tended to occur in regions of the epithelium in which expression of the terminal differentiation protein involucrin was delayed or absent. Thus, K19 expression may be linked to the retention of stem cell character or a state otherwise uncommitted to terminal squamous differentiation. Suprabasal K19 staining is clearly correlated with premalignant change in oral epithelium and therefore promises to be a useful tool in oral histopathologic diagnosis.