Generation of functional eggs and sperm from cryopreserved whole testes
Generation of functional eggs and sperm from cryopreserved whole testes
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DOI：
10.1073/pnas.1218468110
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发表时间：
2013-01-29
影响因子：
11.1
通讯作者：
Yoshizaki, Goro
中科院分区：
文献类型：
--
作者：
Lee, Seungki;Iwasaki, Yoshiko;Yoshizaki, Goro
保护濒危鱼类至关重要。冷冻银行可以提供一个有效的备份措施，用于与自然种群的保护，但是，冷冻保存的鱼卵和胚胎的方法尚未开发。本研究建立了一种从冷冻的A型精原细胞（ASG）中获得功能性卵子和精子的方法。从虹鳟鱼的整个睾丸缓慢冷冻在cryomedium，和这些睾丸内的ASGs的生存能力并没有减少超过728天的冷冻期。冻融ASG腹腔内移植到无菌三倍体孵化迁移，并纳入，受体生殖嵴。ASGs的可移植性并没有下降后，多达939天的冷冻保存。近一半的三倍体受体产生了来自冷冻ASG的功能性卵子或精子，并显示出高繁殖力。所得配子的受精导致正常的冷冻ASG衍生后代的成功生产。这种方法的可行性和简单性，将呼吁立即应用于真实的保护濒危野生鲑。
The conservation of endangered fish is of critical importance. Cryobanking could provide an effective backup measure for use in conjunction with the conservation of natural populations; however, methodology for cryopreservation of fish eggs and embryos has not yet been developed. The present study established a methodology capable of deriving functional eggs and sperm from frozen type A spermatogonia (ASGs). Whole testes taken from rainbow trout were slowly frozen in a cryomedium, and the viability of ASGs within these testes did not decrease over a 728-d freezing period. Frozen-thawed ASGs that were intraperitoneally transplanted into sterile triploid hatchlings migrated toward, and were incorporated into, recipient genital ridges. Transplantability of ASGs did not decrease after as much as 939 d of cryopreservation. Nearly half of triploid recipients produced functional eggs or sperm derived from the frozen ASGs and displayed high fecundity. Fertilization of resultant gametes resulted in the successful production of normal, frozen ASG-derived offspring. Feasibility and simplicity of this methodology will call for an immediate application for real conservation of endangered wild salmonids.