Discrimination between Bifidobacterium species from human and animal origin by PCR-restriction fragment length polymorphism
Discrimination between Bifidobacterium species from human and animal origin by PCR-restriction fragment length polymorphism
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DOI：
10.4315/0362-028x-67.6.1284
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发表时间：
2004-06-01
影响因子：
2
通讯作者：
Daube, G
中科院分区：
文献类型：
--
作者：
Delcenserie, V;Bechoux, N;Daube, G
双歧杆菌是人类和动物的正常肠道菌群。双歧杆菌属包括 31 个具有显着宿主特异性的物种。考虑到它们的特性，我们建议使用双歧杆菌作为粪便污染指标。 16S rDNA 基因上的 PCR 限制性片段长度多态性用于区分不同的双歧杆菌物种。使用一种或两种限制性内切酶将属于 13 个不同物种的 64 种菌株与动物或人类来源区分开来。此外，所使用的引物是双歧杆菌属的特异性引物。因此，该方法可以确定样品中双歧杆菌的存在及其污染源。
Bifidobacteria are normal intestinal flora in humans and animals. The genus Bifidobacterium includes 31 species of significant host specificity. Taking into account their properties, we proposed to use bifidobacteria as fecal contamination indicators. PCR-restriction fragment length polymorphism on the 16S rDNA gene was used to distinguish the different Bifidobacterium species. Sixty-four strains belonging to 13 different species were differentiated from animal or human origin using one or two restriction enzymes. Moreover, the primers used were specifics of the Bifidobacterium genus. Therefore, this method made it possible to determine both the presence of bifidobacteria in a sample and its origin of contamination.