Expression of aldose reductase and sorbitol dehydrogenase genes in Schwann cells isolated from rat: effects of high glucose and osmotic stress
Expression of aldose reductase and sorbitol dehydrogenase genes in Schwann cells isolated from rat: effects of high glucose and osmotic stress
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DOI：
10.1016/s0169-328x(01)00009-2
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发表时间：
2001-03-05
期刊：
影响因子：
--
通讯作者：
Yabe-Nishimura, C
中科院分区：
文献类型：
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作者：
Maekawa, K;Tanimoto, T;Yabe-Nishimura, C
为了研究雪旺细胞多元醇途径的活性，我们采用竞争性RT-PCR技术检测了高糖和高渗条件下培养的雪旺细胞中醛糖还原酶（AR）和山梨醇脱氢酶（SDH）的mRNA水平。施旺细胞中AR和SDH mRNA的表达不受培养基中高葡萄糖含量（30 mM）的影响。另一方面，渗透胁迫引起AR mRNA的显着增加，而对SDH mRNA的表达没有任何影响。AR mRNA的水平，通过该RT-PCR系统确定与AR活性，以及在高渗条件下培养的雪旺细胞中积累的山梨醇的水平显着相关。这些研究结果表明，在相反的AR表达的诱导渗透压，高葡萄糖本身不上调表达的酶构成的多元醇途径在雪旺细胞。本研究建立的RT-PCR系统可能是一个有用的工具，在确定AR和SDH的相对贡献的代谢紊乱导致糖尿病并发症。(C)2001 Elsevier Science B. V.保留所有权利。
To investigate the polyol pathway activity in Schwann cells, we determined the mRNA levels of aldose reductase (AR) and sorbitol dehydrogenase (SDH) in cultured cells under hyperglycemic or hyperosmotic conditions using competitive RT-PCR technique. The expressions of AR and SDH mRNAs in Schwann cells were unaltered by high (30 mM) glucose content in the medium. On the other hand, osmotic stress elicited significant increases in AR mRNA without any effect on SDH mRNA expression. The levels of AR mRNA determined by this RT-PCR system were significantly correlated with AR activity, as well as the levels of sorbitol accumulated in Schwann cells cultured under hyperosmotic conditions. These findings suggest that in contrast to the induction of AR expression by osmotic stress, high glucose per se does not up-regulate expression of the enzymes constituting the polyol pathway in Schwann cells. The RT-PCR system developed in this study may be a useful tool in ascertaining the relative contributions of AR and SDH to the metabolic derangements leading to diabetic complications. (C) 2001 Elsevier Science B.V. All rights reserved.