Early posthatch starvation decreases satellite cell proliferation and skeletal muscle growth in chicks
Early posthatch starvation decreases satellite cell proliferation and skeletal muscle growth in chicks
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DOI：
10.1093/jn/130.4.858
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发表时间：
2000-04-01
影响因子：
4.2
通讯作者：
Sklan, D
中科院分区：
文献类型：
--
作者：
Halevy, O;Geyra, A;Sklan, D
研究了孵化后饥饿对雏鸡骨骼肌生长和卫星细胞增殖的影响。孵化后将雏鸡饲喂或饥饿 48 小时（d 0-d 2、d 2-d 4 或 d 4-d 6），然后再饲喂 41 d。在整个实验过程中，饥饿雏鸡的身体和胸肌重量明显低于喂食对照雏鸡。组织化学染色显示，饥饿组的骨骼肌纤维发育落后于进食组。孵化后第 2 至 4 天和第 4 至 6 天饥饿的影响逐渐小于孵化后立即饥饿的影响（P < 0.05）。胸肌卫星细胞的体外培养表明，喂食雏鸡的DNA合成和每克肌肉的细胞数量在第2天和第3天达到峰值，然后下降。相比之下，饥饿雏鸡细胞中的 DNA 合成在第 2 天下降，在第 3 天重新喂食后增加。每克肌肉的细胞数量也出现了类似的模式。然而，总的来说，饥饿组的细胞数量往往高于对照组（P < 0.1）。用培养细胞获得的结果与实验鸡胸肌中5-溴-2'-脱氧尿苷和增殖细胞核抗原的原位免疫染色以及生长激素受体表达平行。这些结果表明卫星细胞培养物是评估产后鸡肌肉生长的可靠工具。我们的结论是，产后立即充足的饲料对于卫星细胞增殖和骨骼肌发育至关重要，因此对于最佳肌肉生长也很重要。
The effect of posthatch starvation on skeletal muscle growth and satellite cell proliferation was examined in chicks. Chicks were either fed or starved for 48 h posthatch (d 0-d 2, d 2-d 4 or d 4-d 6) and then refed for 41 d. Body and breast muscle weights were significantly lower in starved chicks than in fed controls throughout the experiment. Histochemical staining revealed that skeletal muscle fiber development in the starved group lagged behind that of the fed group. Starvation from d 2 to 4 and d 4 to 6 posthatch had a progressively lesser effect than did immediate posthatch starvation (P < 0.05). in vitro culturing of breast muscle satellite cells revealed that DNA synthesis and number of cells per gram of muscle in the fed chicks peaked on d 2 and d 3, and then declined. In contrast, DNA synthesis in the cells of starved chicks declined on d 2 and increased on d 3 when chicks were refed. A similar pattern was seen for the number of cells per gram muscle; however, in general cell numbers tended to be higher in the starved group than in controls (P < 0.1). The results obtained with cultured cells were parallel with in situ immunostaining with 5-bromo-2' -deoxyuridine and proliferating cell nuclear antigen in breast muscle from experimental chicks, and with growth hormone receptor expression. These results suggest that satellite cell cultures are a reliable tool for evaluating muscle growth in postnatal chickens. We conclude that sufficient feed in the immediate postnatal period is critical for satellite cell proliferation and skeletal muscle development and is thus important for optimal muscle growth.