Methods for Measuring Bioactive Molecules in Blood by a Smartphone Using Bioluminescent Ratiometric Indicators
Methods for Measuring Bioactive Molecules in Blood by a Smartphone Using Bioluminescent Ratiometric Indicators
复制标题
使用生物发光比率指示器通过智能手机测量血液中生物活性分子的方法
DOI：
10.1007/978-1-0716-2473-9_16
复制
发表时间：
2022
影响因子：
--
通讯作者：
Nagai T
中科院分区：
文献类型：
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作者：
Hattori M;Itoh Y;Nagai T
生物发光指示剂有助于以高灵敏度测定血液样品中的生物活性分子。使用明亮的荧光素酶，它的生物发光（BL）可以很容易地检测到传统的光传感设备。在本章中，我们描述了一种通过智能手机摄像头拍摄BL图像来测量血液中生物活性分子的协议。我们使用比率生物发光UCBR指示剂BABI（生物发光指示剂胆红素评估）和智能手机摄像头测量小鼠血液中的未结合胆红素（UCBR）浓度。我们表明UCBR浓度很容易通过用智能手机相机测量BL颜色的方差来确定。这种方法提供了一种实用的方法，以导致未来的床旁诊断与快速和简单的程序。
Bioluminescent indicators facilitate determination of bioactive molecules in blood samples with high sensitivity. Using a bright luciferase, its bioluminescence (BL) can be easily detected by conventional light sensing devices. In this chapter, we describe a protocol to measure bioactive molecules in blood by taking the BL images with a smartphone camera. We exemplify the measurement of unconjugated bilirubin (UCBR) concentration in the blood of mice using a ratiometric bioluminescent UCBR indicator, BABI (bilirubin assessment with a bioluminescent indicator), and a smartphone camera. We show the UCBR concentration is easily determined through measuring the variance in the BL color with a smartphone camera. This method provides a practical method to lead to future point-of-care diagnosis with quick and simple procedures.