Interlaboratory Evaluation of a Portable Fluorescence Method for the Measurement of Trace Beryllium in the Workplace
Interlaboratory Evaluation of a Portable Fluorescence Method for the Measurement of Trace Beryllium in the Workplace
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用于测量工作场所痕量铍的便携式荧光方法的实验室间评估
DOI：
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
A. Agrawal
中科院分区：
文献类型：
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作者：
K. Ashley;T. Mccleskey;M. Brisson;G. Goodyear;J. Cronin;A. Agrawal
洛斯阿拉莫斯国家实验室（Los Alamos National Laboratory， LANL）的研究人员开发了一种现场便携式荧光法，用于测量工作场所样品（如表面灰尘和空气过滤器）中的痕量铍。该技术已获得私人许可，并可用于商业用途。我们与铍健康和安全委员会分析小组委员会合作，对LANL现场便携式荧光法进行了实验室间合作评价。进行实验室间研究的目的是提供可用于支持标准方法的性能数据。混合纤维素酯（MCE）膜过滤器和Whatman 541过滤器加入铍标准溶液，使过滤器的范围为≈0.05 -≈0.5µg Be每个样品。然后对这些过滤器进行编码（以确保盲分析）并送到参与的实验室，在那里对它们进行分析。分析包括以下步骤：1。从运输盒中取出过滤器，并将其放入15毫升离心管中；2. 机械辅助提取过滤器在5ml的1%二氟化铵溶液（水溶液）中30分钟；3. - 4。样品提取液等分（100µL）过滤并转移到荧光皿中；5. 引入1.9 mL检测溶液（以影响荧光试剂与提取样品中的铍的反应）；和6。使用便携式荧光计测量≈475 nm处的荧光。这项工作提供了性能数据，以支持作为国家职业安全与健康研究所（NIOSH）方法和ASTM国际标准出版的程序。
Researchers at Los Alamos National Laboratory (LANL) developed a field-portable fluorescence method for the measurement of trace beryllium in workplace samples such as surface dust and air filters. The technology has been privately licensed and is commercially available. In cooperation with the Analytical Subcommittee of the Beryllium Health and Safety Committee, we have carried out a collaborative interlaboratory evaluation of the LANL field-portable fluorescence method. The interlaboratory study was conducted for the purpose of providing performance data that can be used to support standard methods. Mixed cellulose ester (MCE) membrane filters and Whatman 541 filters were spiked with beryllium standard solutions so that the filters spanned the range ≈0.05 - ≈0.5 µg Be per sample. Sets of these filters were then coded (to ensure blind analysis) and sent to participating laboratories, where they were analyzed. Analysis consisted of the following steps: 1. Removal of the filters from transport cassettes and placement of them into 15-mL centrifuge tubes; 2. mechanically- assisted extraction of the filters in 5 mL of 1 % ammonium bifluoride solution (aqueous) for 30 min; 3.-4. filtration and transfer of sample extract aliquots (100 µL) into fluorescence cuvettes; 5. introduction of 1.9 mL of detection solution (to effect reaction of the fluorescence reagent with beryllium in the extracted sample); and 6. measurement of fluorescence at ≈475 nm using a portable fluorometer. This work presents performance data in support of a procedure that is targeted for publication as a National Institute for Occupational Safety and Health (NIOSH) method and as an ASTM International standard.