Profiling food volatiles by comprehensive two-dimensional gas chromatography coupled with mass spectrometry: Advanced fingerprinting approaches for comparative analysis of the volatile fraction of roasted hazelnuts (Corylus avellana L.) from different origins
Profiling food volatiles by comprehensive two-dimensional gas chromatography coupled with mass spectrometry: Advanced fingerprinting approaches for comparative analysis of the volatile fraction of roasted hazelnuts (Corylus avellana L.) from different origins
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DOI：
10.1016/j.chroma.2010.07.006
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发表时间：
2010-09-10
影响因子：
4.1
通讯作者：
Tao, Qingping
中科院分区：
文献类型：
--
作者：
Cordero, Chiara;Liberto, Erica;Tao, Qingping
这项研究考察了先进的指纹方法(即非靶标方法)如何根据样品组在GC x GC色谱面上的成分分布来提供关于样品组的可靠和特定的信息。采用顶空气相固相微萃取技术(HS-SPME)对9个不同产地、风味和质地不同的烘焙榛子(Corylus Avellana L)的挥发性成分进行了采样，并用GC-GC-QMS对其进行了分析。所得到的模式通过以下方式处理：(A)“色谱指纹”，即基于保留时间标准的模式识别程序，其中通过覆盖色谱面的综合峰模式来建立峰对应；以及(B)具有可靠峰匹配的“全面模板匹配”，其中峰对应受保留时间和MS碎裂模式相似性标准的限制。指纹图谱结果表明，通过在样品中包括所有检测到的成分的比较和关联，可以如何提高GC x GC的分辨潜力，此外，通过定位具有重要作用的化合物，还可以在比较分析中提供可靠的结果。结果是通过挥发物的化学形态来完成的，样品轮廓被扩展到已知的标记，其分布可以与感官特性、地理来源或热处理对不同类别化合物的影响相关。这里提出的数据解释的综合方法可能有助于通过与感官特性和来源严格且一致地关联的可测量参数来评估产品的特异性和质量。(C)2010爱思唯尔B.V.保留所有权利。
This study examined how advanced fingerprinting methods (i.e., non-targeted methods) provide reliable and specific information about groups of samples based on their component distribution on the GC x GC chromatographic plane. The volatile fractions of roasted hazelnuts (Corylus avellana L) from nine different geographical origins, comparably roasted for desirable flavor and texture, were sampled by headspace-solid phase micro extraction (HS-SPME) and then analyzed by GC x GC-qMS. The resulting patterns were processed by: (a) "chromatographic fingerprinting", i.e., a pattern recognition procedure based on retention-time criteria, where peaks correspondences were established through a comprehensive peak pattern covering the chromatographic plane; and (b) "comprehensive template matching" with reliable peak matching, where peak correspondences were constrained by retention time and MS fragmentation pattern similarity criteria. Fingerprinting results showed how the discrimination potential of GC x GC can be increased by including in sample comparisons and correlations all the detected components and, in addition, provide reliable results in a comparative analysis by locating compounds with a significant role. Results were completed by a chemical speciation of volatiles and sample profiling was extended to known markers whose distribution can be correlated to sensory properties, geographical origin, or the effect of thermal treatment on different classes of compounds. The comprehensive approach for data interpretation here proposed may be useful to assess product specificity and quality, through measurable parameters strictly and consistently correlated to sensory properties and origin. (C) 2010 Elsevier B.V. All rights reserved.