SIGNAL TRANSDUCTION IN HUMAN MONOCYTES AND GRANULOCYTES THROUGH THE PI-LINKED ANTIGEN CD14
SIGNAL TRANSDUCTION IN HUMAN MONOCYTES AND GRANULOCYTES THROUGH THE PI-LINKED ANTIGEN CD14
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DOI：
10.1016/0014-5793(90)81049-t
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发表时间：
1990-10-29
期刊：
影响因子：
3.5
通讯作者：
BJERKNES, R
中科院分区：
文献类型：
--
作者：
LUNDJOHANSEN, F;OLWEUS, J;BJERKNES, R
研究了 Pi 连接的 CD14 分子在人单核细胞和粒细胞信号介导中的可能作用。使用流式细胞术和荧光指示剂 Fluo-3 和二氢罗丹明-123 结果表明，CD14 分子的交联会诱导单核细胞和粒细胞细胞质钙浓度以及单核细胞 H2O2 产生的增加。发现这些反应独立于 IgG Fc 受体，表明 CD 14 分子具有内在的信号介导能力。
A possible role for the Pi-linked CD14 molecule in human monocyte and granulocyte signal mediation was investigated. Using flow cytometry and the fluorescent indicators Fluo-3 and dihydrorhodamine-123 it was shown that crosslinking of the CD14 molecule induces an increase in monocyte and granulocyte cytoplasmic calcium concentration and monocyte H2O2production. These responses were found to be independent of IgG Fc receptors and suggest an intrinsic signal mediating capacity of the CD 14 molecule.