Osteoblast response and calcium deposition on phospholipid modified surfaces.
Osteoblast response and calcium deposition on phospholipid modified surfaces.
复制标题
成骨细胞反应和磷脂修饰表面上的钙沉积。
DOI：
10.1023/b:jmsm.0000030211.32655.80
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Satsangi,RajivK
中科院分区：
文献类型：
--
作者：
Satsangi,Neera;Satsangi,Arpan;Glover,Renee;Ong,JooL;Satsangi,RajivK
In this study, the effect of calcium phosphate complexed phospholipid (Ca–PL–PO4) coatings on solid surfaces on the in vitro calcium (Ca) deposition and on the osteoblast responses was evaluated. Commercially available phospholipids were converted to their Ca–PL–PO4, and were coated on glass Petri dishes. The coated dishes were immersed in the simulated body fluid for up to 14 days under sterilized conditions at 37 °C, and the amount of calcium (Ca) deposited was quantified. Similarly, by measuring the alkaline phosphatase specific activity, the differentiation of osteoblast precursor cells were evaluated after seeding the cells on Ca–PL–PO4coated cell culture plastics. It was observed that all Ca–PL–PO4enhanced Ca deposition on coated surfaces. The, polar head group of phospholipids in coated surfaces was observed to have an influence on the Ca deposition as well as the osteoblast differentiation. Among the phospholipids evaluated, phosphatidylserine (Ca–PS–PO4) exhibited the strongest calcium deposition and more enhanced alkaline phosphatase specific activity. It was therefore concluded from this study that Ca–PS–PO4surface modification may be an alternative method for enhancing bone–implant interactions.
