CONTROL OF PLASTID GENE-EXPRESSION DURING DEVELOPMENT - THE LIMITED ROLE OF TRANSCRIPTIONAL REGULATION
CONTROL OF PLASTID GENE-EXPRESSION DURING DEVELOPMENT - THE LIMITED ROLE OF TRANSCRIPTIONAL REGULATION
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DOI：
10.1016/0092-8674(87)90290-x
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发表时间：
1987-05-08
期刊：
影响因子：
64.5
通讯作者：
GRUISSEM, W
中科院分区：
文献类型：
--
作者：
DENG, XW;GRUISSEM, W
我们分析了在光照下菠菜子叶叶绿体发育和叶片形成过程中，叶绿体基因的转录调控。由叶绿体基因编码的RNA在发育过程中以不同的动力学方式积累。使用一种新的叶绿体连续转录实验，我们证明了一大组不同的叶绿体基因的转录调控对于控制它们的表达相对来说是次要的。叶绿体基因组的总转录活性在光照后增加，在叶片发育过程中降低。这种对一般转录活性的调节同时影响大多数叶绿体基因，并且与叶绿体DNA拷贝数的调节无关。不同基因的相对转录活性没有大的变化，尽管它们的稳态mRNA水平发生了显著变化。对10个特定的叶绿体基因的分析表明，它们的相对转录活性在整个发育过程中基本保持不变。这种有限的转录调控表明，高等植物中的叶绿体基因表达在转录后水平上得到了有效的控制。
We have analyzed the transcriptional regulation of plastid genes during chloroplast development in illuminated spinach cotyledons and during leaf formation. The RNAs encoded by plastid genes accumulate with different kinetics during the developmental transitions. Using a novel plastid run-on transcription assay we demonstrate that the transcriptional regulation of a large, diverse group of chloroplast genes is of relatively minor importance for the control of their expression. The general transcriptional activity of the plastid genome increases after illumination and decreases during leaf development. This modulation of general transcriptional activity affects most plastid genes simultaneously and is not correlated with adjustments of the plastid DNA copy number. There are no major changes in the relative transcriptional activities of different genes, although their steady-state mRNA levels change dramatically. The analysis of ten specific plastid genes shows that their relative transcriptional activities are largely maintained throughout the developmental program. This limited transcriptional regulation suggests that plastid gene expression in higher plants is effectively controlled at the posttranscriptional level.