Impaired IFN-γ-secreting capacity in mycobacterial antigen-specific CD4 T cells during chronic HIV-1 infection despite long-term HAART
Impaired IFN-γ-secreting capacity in mycobacterial antigen-specific CD4 T cells during chronic HIV-1 infection despite long-term HAART
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DOI：
10.1097/01.aids.0000218545.31716.a4
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发表时间：
2006-04-04
期刊：
影响因子：
3.8
通讯作者：
Dorrell, L
中科院分区：
文献类型：
--
作者：
Sutherland, R;Yang, HB;Dorrell, L
目的：为了确定慢性HIV-1感染的长期HAART是否恢复完全功能的结核分枝杆菌（MTB）特异性CD 4 T细胞应答。设计：一项对连续HAART治疗超过一年的HIV-1血清阳性受试者（CD 4细胞计数大于300个细胞/μ l且未检测到病毒血症）、原发性HIV-1感染（PHI）的抗逆转录病毒初治个体进行的横断面研究，方法：通过酶联免疫斑点试验和细胞内细胞因子染色离体定量纯化的蛋白衍生物（PPD）特异性分泌精氨酸的CD 4 T细胞。结果：慢性HAART治疗的HIV-1阳性和PHI患者的PPD特异性IFN-γ分泌性CD 4 T细胞明显低于健康对照组[中位数分别为30、155和582个斑点形成细胞/百万外周血单个核细胞（PBMC），P
Objective: To determine whether long-term HAART in chronic HIV-1 infection restores fully functional Mycobacterium tuberculosis (MTB)-specific CD4 T-cell responses.Design: A cross-sectional study of HIV-1-seropositive subjects on continuous HAART for over one year with CD4 cell counts greater than 300 cells/mu l and undetectable viraemia, antiretroviral-naive individuals with primary HIV-1 infection (PHI), and healthy bacillus Calmette-Guerin-vaccinated low-risk controls.Methods: Purified protein derivative (PPD)-specific cytokine-secreting CD4 T cells were quantified ex vivo by enzyme-linked immunospot assay and intracellular cytokine staining. Lymphoproliferation was detected by [H-3]-thymidine incorporation.Results: PPD-specific IFN-gamma-secreting CD4 T cells were markedly reduced in chronic HAART-treated HIV-1-positive and PHI subjects compared with healthy controls [medians 30, 155 and 582 spot-forming cells/million peripheral blood mononuclear cells (PBMC), respectively, P