SPM43.1 Contributes to Acid-Resistance of Non-Symplasmata-Forming Cells in Pantoea agglomerans YS19
SPM43.1 Contributes to Acid-Resistance of Non-Symplasmata-Forming Cells in Pantoea agglomerans YS19
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SPM43.1 有助于成团泛菌 YS19 中非合浆体形成细胞的耐酸性
DOI：
10.1007/s00284-011-0055-6
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发表时间：
2011
影响因子：
2.6
通讯作者：
Yongjun Feng
中科院分区：
文献类型：
--
作者：
Qianqian Li;Yuxuan Miao;Ting Yi;Jia Zhou;Zhenyue Lu;Yongjun Feng
成团泛菌（Pantoea agglomeransYS 19）是一株水稻内生细菌，其特征是形成多细胞生物膜状结构，称为共质体。共质体形成细胞和增殖细胞之间的表型差异对于理解YS 19的生存策略至关重要。在这项研究中，一个43.1 kDa的蛋白质SPM 43.1被鉴定为显示出显着的抗性，由变性酸性条件引起的聚集效应。MALDI-TOF分析数据表明它是麦芽糖结合蛋白同系物，同时含有与伴侣蛋白ClpB同源的序列。在酸性条件下，SPM43.1蛋白具有分子伴侣样活性，圆二色谱显示其构象从有序结构转变为无序结构，与大多数分子伴侣蛋白具有相似的性质。当细菌细胞开始聚集时，SPM 43.1在YS 19中开始表达，但其在共生细胞中的量大大超过在共质体形成细胞中的量，这表明其对共生细胞在经历环境波动时的存活起着至关重要的作用。然而，该细菌倾向于形成共质体，而不表达SPM 43.1蛋白，以在人工设置的波动（此处为酸性）环境中存活。这项研究提供了有价值的信息的生活方式和生存策略的微生物，形成多细胞聚集体在特定的生长阶段。
Pantoea agglomeransYS19 is a rice endophytic bacterium characterized to form multicellular biofilm-like structures called symplasmata. Phenotypic distinctions between symplasmata-forming cells and planktonic cells are crucial for understanding YS19’s survival strategies. In this study, a 43.1 kDa protein SPM43.1 was identified to show significant resistance to the aggregation effect caused by denaturing acidic conditions. MALDI-TOF analysis data indicated that it is a maltose-binding protein homolog while contains sequence homologous to the chaperone protein, ClpB. The purified SPM43.1 protein was detected to exhibit chaperone-like activities at acidic conditions, where its conformation transformed from an ordered to a globally less ordered structure as revealed by circular dichroism spectroscopy, showing a similar property to most chaperone proteins. The expression of SPM43.1 in YS19 is initiated when bacterial cells begin to aggregate, yet its amount in planktonic cells greatly exceeds that in symplasmata-forming cells, suggesting its crucial role to the survival of planktonic cells in experiencing environmental fluctuations. However, the bacterium prefers to form symplasmata, while not to express SPM43.1 proteins, for surviving the artificially set fluctuant (acid here) environments. This study provides valuable information on the life styles and survival strategies of microorganisms that forms multicellular aggregates at specific growth stages.
影响因子：
3.2
作者：
Capestany, Cindy A.;Tribble, Gena D.;Lamont, Richard J.
通讯作者：
Lamont, Richard J.