One-pot d-lactic acid production using undetoxified acid-pretreated corncob slurry by an adapted Pediococcus acidilactici.
One-pot d-lactic acid production using undetoxified acid-pretreated corncob slurry by an adapted Pediococcus acidilactici.
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DOI：
10.1016/j.biortech.2022.127993
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发表时间：
2022-09
影响因子：
11.4
通讯作者：
Zhongyang Qiu;Xushen Han;Jianlong He;Yanan Jiang;Guangli Wang;Zejia Wang;Xiaoyan Liu;Jun Xia;Ning Xu;Ai-yong He;Hanqi Gu;Jiaxing Xu
中科院分区：
文献类型：
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作者：
Zhongyang Qiu;Xushen Han;Jianlong He;Yanan Jiang;Guangli Wang;Zejia Wang;Xiaoyan Liu;Jun Xia;Ning Xu;Ai-yong He;Hanqi Gu;Jiaxing Xu
Inhibitor tolerance is still a bottleneck for lactic acid bacteria in lignocellulose biorefinery, while it is hard to obtain one engineered strain with strong tolerance to all inhibitors. Herein, a robust adaptedd-lactic acid producing strainPediococcus acidilacticiXH11 was obtained by 111 days’ long-term adaptive evolution in undetoxified corncob prehydrolysates. The adapted strain had higher inhibitors tolerance compared to the parental strain, primarily due to its increased conversion capacities of four typical aldehyde inhibitors (furfural, HMF, vanillin, and 4-hydroxybenzaldehyde). One-pot simultaneous saccharification and co-fermentation was successfully achieved using the whole slurry of acid-pretreated corncob without solid–liquid separation and detoxification, by applying the adaptedP. acidilacticiXH11. Finally, 61.9 g/L ofd-lactic acid was generated after 96 h’ fermentation (xylose conversion of 89.9 %) with the overall yield of 0.48 g/g dry corncob. This study gave an important option for screening of industrial strains in cellulosic lactic acid production processes.
