A clinical instrument for combined raman spectroscopy-optical coherence tomography of skin cancers.
A clinical instrument for combined raman spectroscopy-optical coherence tomography of skin cancers.
复制标题
用于皮肤癌联合拉曼光谱-光学相干断层扫描的临床仪器。
DOI：
10.1002/lsm.21041
复制
发表时间：
2011
影响因子：
2.4
通讯作者：
Mahadevan-Jansen,Anita
中科院分区：
文献类型：
--
作者：
Patil,ChetanA;Kirshnamoorthi,Harish;Ellis,DarrelL;vanLeeuwen,TonG;Mahadevan-Jansen,Anita
背景和目的当前皮肤癌的诊断标准是目视检查，然后进行活检和组织病理学检查。这个过程可能是侵入性的、主观的、耗时且昂贵的。光学相干断层扫描 (OCT) 和拉曼光谱 (RS) 等光学技术已被开发用于根据疾病的形态或生化特征对皮肤病变进行非侵入性表征。这项工作的目的是报告一种能够通过 RS-OCT 对皮肤癌进行形态学和生化表征的临床仪器。材料和方法该便携式仪器采用独立的 785 nm RS 和 1,310 nm OCT 系统主干。这两种模式集成在 4″ (H) × 5″(W) × 8″(L) 临床探头中。该探头能够连续采集共同配准的 OCT 和 RS 数据集。使用散射体模估计皮肤中 RS 集合的轴向响应。此外，还报告了来自癌症和非癌症病变患者的 RS-OCT 数据。 结果 RS-OCT 仪器能够使用 OCT 以高达 8 帧/秒的速度筛查大至 15 毫米（横向）× 2.4 毫米（深度）的区域，并确定执行 RS 的位置。 RS 信号是从深度约为 530μm 的 44μm 横向光斑收集的。据报道，来自浅表疤痕和结节性基底细胞癌的 RS-OCT 数据集证明了该仪器的临床潜力。结论此处报道的 RS-OCT 仪器能够在临床环境中对癌性皮肤病变进行形态学和生化表征。 OCT 可以显示微观结构不规则性并对病变进行初步形态分析。这些图像可用于指导生化特定拉曼光谱的采集。然后可以对这两个数据集进行相互评估，以利用 RS 和 OCT 的互补性质。激光外科。医学。 42:143–151, 2011 © 2011 Wiley-Liss, Inc.
Background and ObjectiveThe current standard for diagnosis of skin cancers is visual inspection followed by biopsy and histopathology. This process can be invasive, subjective, time consuming, and costly. Optical techniques, including Optical Coherence Tomography (OCT) and Raman Spectroscopy (RS), have been developed to perform non‐invasive characterization of skin lesions based on either morphological or biochemical features of disease. The objective of this work is to report a clinical instrument capable of both morphological and biochemical characterization of skin cancers with RS‐OCT.Materials and MethodsThe portable instrument utilizes independent 785 nm RS and 1,310 nm OCT system backbones. The two modalities are integrated in a 4″ (H) × 5″(W) × 8″(L) clinical probe. The probe enables sequential acquisition of co‐registered OCT and RS data sets. The axial response of the RS collection in the skin was estimated using scattering phantoms. In addition, RS‐OCT data from patients with cancerous and non‐cancerous lesions are reported.ResultsThe RS‐OCT instrument is capable of screening areas as large as 15 mm (transverse) by 2.4 mm (in depth) at up to 8 frames/second with OCT, and identifying locations to perform RS. RS signal is collected from a 44 µm transverse spot through a depth of approximately 530 µm. RS‐OCT data sets from a superficial scar and a nodular BCC are reported to demonstrate the clinical potential of the instrument.ConclusionThe RS‐OCT instrument reported here is capable of morphological and biochemical characterization of cancerous skin lesions in a clinical setting. OCT can visualize microstructural irregularities and perform an initial morphological analysis of the lesion. The images can be used to guide acquisition of biochemically specific Raman spectra. The two data sets can then be evaluated with respect to one another to take advantage of the mutually complimentary nature of RS and OCT. Lasers Surg. Med. 42:143–151, 2011 © 2011 Wiley‐Liss, Inc.