Mesalamine-Mediated Amelioration of Experimental Colitis in Piglets Involves Gut Microbiota Modulation and Intestinal Immune Cell Infiltration.
Mesalamine-Mediated Amelioration of Experimental Colitis in Piglets Involves Gut Microbiota Modulation and Intestinal Immune Cell Infiltration.
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美沙拉胺介导的实验性仔猪结肠炎的改善涉及肠道菌群调节和肠道免疫细胞浸润。
DOI：
10.3389/fimmu.2022.883682
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发表时间：
2022
影响因子：
7.3
通讯作者：
中科院分区：
文献类型：
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作者：
美沙拉嗪（MES），也称为5-氨基水杨酸，可有效治疗轻度至中度溃疡性结肠炎（UC）。其作用机制尚未完全阐明。本研究旨在研究MES治疗对葡聚糖硫酸钠（DSS）诱导的断奶仔猪UC模型中肠道微生物群和免疫系统的影响。将18头断奶仔猪随机分为对照组（CON，蒸馏水）、DSS组（DSS，3%DSS）和MES组（MES，3%DSS +2g/天MES）。我们的研究结果表明，MES治疗减轻了DSS诱导的仔猪结肠炎，表现为腹泻指数评分降低和平均日增重增加（P < 0.05）。二胺氧化酶活性、D-乳酸水平降低（P < 0.05），粘膜损伤减轻。MES治疗还降低了DSS诱导的结肠炎仔猪中产甲烷菌的丰度，并减少了结肠CD 11b+巨噬细胞和CD 3 + T细胞的浸润（P < 0.05）。总的来说，这些数据表明MES治疗介导的结肠炎保护可能涉及微生物群和免疫细胞的改变。
Mesalamine (MES), also known as 5-aminosalicylic acid, is effective in treating mild to moderate ulcerative colitis (UC). The mechanisms of its actions are not fully elucidated. The aim of this study was to investigate the effects of MES treatment on intestinal microbiota and immune system in an dextran sulfate sodium (DSS)-induced UC model in postweaning piglets. Eighteen weaned piglets were assigned randomly to the following treatments: control group (CON, distilled water), DSS group (DSS, 3% DSS), and MES group (MES, 3% DSS + 2 g/day MES). Our results showed that MES treatment alleviates DSS-induced colitis in piglets, as evidenced by a reduced diarrhea index score and increased average daily gain (P < 0.05). This is accompanied by decreased diamine oxidase activity, D-lactate level (P < 0.05), and attenuated mucosal damage. MES treatment also decreased the abundance of Methanogens and reduced colon CD11b+ macrophage and CD3+ T-cell infiltrations in piglets with DSS-induced colitis (P < 0.05). Collectively, these data indicate that MES treatment-mediated colitis protection may involve microbiota and immune cell alterations.