Bleomycin-induced DNA repair by Saccharomyces cerevisiae ATP-dependent polydeoxyribonucleotide ligase.
Bleomycin-induced DNA repair by Saccharomyces cerevisiae ATP-dependent polydeoxyribonucleotide ligase.
复制标题
博莱霉素通过酿酒酵母 ATP 依赖性多脱氧核糖核苷酸连接酶诱导 DNA 修复。
DOI：
10.1128/jb.170.10.4991-4994.1988
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发表时间：
1988
影响因子：
3.2
通讯作者：
Moore,CW
中科院分区：
文献类型：
--
作者：
Moore,CW
与连接酶缺陷型（cdc 9）酿酒酵母（Saccharomyces cerevisiae）（其不重新连接博来霉素诱导的DNA断裂）相反，连接酶活性型（cdc 9）酵母细胞在处理后90至120分钟内消除了约90%的DNA断裂。实验条件限制了博莱霉素切割的DNA中不寻常的3 '-磷酸乙醇酸末端的酶促去除，并且涉及产生等同数量的DNA断裂的剂量或产生等同杀伤的剂量。
In contrast to ligase-deficient (cdc9) Saccharomyces cerevisiae, which did not rejoin bleomycin-induced DNA breaks, ligase-proficient (CDC9) yeast cells eliminated approximately 90% of DNA breaks within 90 to 120 min after treatment. Experimental conditions restricted enzymatic removal of the unusual 3'-phosphoglycolate termini in DNA cleaved by bleomycin and involved doses producing equivalent numbers of DNA breaks or doses producing equivalent killing.