A single cytosine deletion in the OsPLS1 gene encoding vacuolar-type H+-ATPase subunit A1 leads to premature leaf senescence and seed dormancy in rice.
A single cytosine deletion in the OsPLS1 gene encoding vacuolar-type H+-ATPase subunit A1 leads to premature leaf senescence and seed dormancy in rice.
复制标题
编码液泡型H-ATP酶亚基A1的OsPLS1基因中的单个胞嘧啶缺失导致水稻叶片过早衰老和种子休眠
DOI：
10.1093/jxb/erw109
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发表时间：
2016-04
影响因子：
6.9
通讯作者：
Pan G
中科院分区：
文献类型：
--
作者：
Yang X;Gong P;Li K;Huang F;Cheng F;Pan G
利用定位克隆技术鉴定了水稻中编码VHA-A1的OsPLS1位点。OsPLS1/VHA-A1参与叶片早衰和种子休眠。叶片衰老是一个受多种因素调控的程序化发育过程，但其分子调控机制尚不完全清楚。本研究对一个新的水稻早衰突变体（ospls1）进行了检测。尽管在幼苗早期发育正常，但突变体叶片表现出病变模拟和早期衰老，分蘖后蒸腾速率高。该突变体还表现出由物理（微孔结构缺陷）和生理（脱落酸敏感性）因素引起的种子休眠。使用基于图谱的克隆方法，我们确定编码空泡H+- atp酶亚基A1 （VHA-A1）的OsPLS1基因中的胞嘧啶缺失是OsPLS1突变体表型异常的基础。在OsPSL1突变体及其野生型中，随着年龄依赖性叶片衰老，OsPSL1/VHA-A1转录物水平逐渐下降。osppls1突变体中OsPSL1/VHA- a1基因表达和VHA酶活性均显著降低，这强烈表明正常的ospls1 /VHA- a1基因在水稻叶片衰老中起负调控作用。突变体ospls1具有较高的水杨酸（SA）水平和活性氧（ROS）积累，并通过上调叶片中WRKY基因激活信号转导。与此一致的是，ospls1突变体对外源SA和/或H2O2表现出超敏反应。综上所述，这些结果表明OsPSL1/VAH-A1突变通过ROS和SA信号的联合作用在叶片早衰中起因果作用。综上所述，OsPLS1与水稻叶片衰老和种子休眠有关。
The OsPLS1 locus that encodes VHA-A1 in rice, was identified by map-based cloning. OsPLS1/VHA-A1 is involved in premature leaf senescence and seed dormancy. Leaf senescence is a programmed developmental process orchestrated by many factors, but its molecular regulation is not yet fully understood. In this study, a novel Oryza sativa premature leaf senescence mutant (ospls1) was examined. Despite normal development in early seedlings, the ospls1 mutant leaves displayed lesion-mimics and early senescence, and a high transpiration rate after tillering. The mutant also showed seed dormancy attributable to physical (defect of micropyle structure) and physiological (abscisic acid sensitivity) factors. Using a map-based cloning approach, we determined that a cytosine deletion in the OsPLS1 gene encoding vacuolar H+-ATPase subunit A1 (VHA-A1) underlies the phenotypic abnormalities in the ospls1 mutant. The OsPSL1/VHA-A1 transcript levels progressively declined with the age-dependent leaf senescence in both the ospls1 mutant and its wild type. The significant decrease in both OsPSL1/VHA-A1 gene expression and VHA enzyme activity in the ospls1 mutant strongly suggests a negative regulatory role for the normal OsPLS1/VHA-A1 gene in the onset of rice leaf senescence. The ospls1 mutant featured higher salicylic acid (SA) levels and reactive oxygen species (ROS) accumulation, and activation of signal transduction by up-regulation of WRKY genes in leaves. Consistent with this, the ospls1 mutant exhibited hypersensitivity to exogenous SA and/or H2O2. Collectively, these results indicated that the OsPSL1/VAH-A1 mutation played a causal role in premature leaf senescence through a combination of ROS and SA signals. To conclude, OsPLS1 is implicated in leaf senescence and seed dormancy in rice.