ZntR positively regulates T6SS4 expression in Yersinia pseudotuberculosis
ZntR positively regulates T6SS4 expression in Yersinia pseudotuberculosis
复制标题
ZntR 正向调节假结核耶尔森菌中 T6SS4 的表达
DOI：
10.1007/s12275-017-6540-2
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发表时间：
2017
影响因子：
3
通讯作者：
Shen Xihui
中科院分区：
文献类型：
--
作者：
Wang Tietao;Chen Keqi;Gao Fen;Kang Yiwen;Chaudhry Muhammad Tausif;Wang Zhuo;Wang Yao;Shen Xihui
VI型分泌系统（T6 SS）是在大多数革兰氏阴性菌中发现的广泛且通用的蛋白质分泌系统。T6 SS的研究主要集中在其对宿主细胞的毒力和细菌间的相互作用中，但研究也表明，假结核耶尔森氏菌中的T6 SS 4参与锌离子的获得，以减轻由多种应激源诱导的羟基自由基的积累。通过比较野生型和zntR突变型Y.利用RNA-seq分析发现，T6 SS 4和其他17种生物学过程受到ZntR的调控。ZntR对Y.进一步的研究表明ZntR通过直接结合T6 SS 4的启动子区来调控T6 SS 4。T6 SS 4表达由锌通过ZntR调节，ZntR维持细胞内锌稳态并控制活性氧的浓度以防止氧化应激下的细菌死亡。本研究为锌依赖性转录调控因子T6 SS 4的调控提供了新的认识，为进一步研究T6 SS转运锌的机制奠定了基础。
The type VI secretion system (T6SS) is a widespread and versatile protein secretion system found in most Gram-negative bacteria. Studies of T6SS have mainly focused on its role in virulence toward host cells and inter-bacterial interactions, but studies have also shown that T6SS4 in Yersinia pseudotuberculosis participates in the acquisition of zinc ions to alleviate the accumulation of hydroxyl radicals induced by multiple stressors. Here, by comparing the gene expression patterns of wild-type and zntR mutant Y. pseudotuberculosis cells using RNA-seq analysis, T6SS4 and 17 other biological processes were found to be regulated by ZntR. T6SS4 was positively regulated by ZntR in Y. pseudotuberculosis, and further investigation demonstrated that ZntR regulates T6SS4 by directly binding to its promoter region. T6SS4 expression is regulated by zinc via ZntR, which maintains intracellular zinc homeostasis and controls the concentration of reactive oxygen species to prevent bacterial death under oxidative stress. This study provides new insights into the regulation of T6SS4 by a zinc-dependent transcriptional regulator, and it provides a foundation for further investigation of the mechanism of zinc transport by T6SS.