New Separation Method of High-purity DHA by Supercritical Fluid Chromatography
New Separation Method of High-purity DHA by Supercritical Fluid Chromatography
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DOI：
10.5650/jos1996.48.1169
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发表时间：
1999-10
期刊：
影响因子：
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通讯作者：
M. Yamaguchi;Yasuhiko Kadota;Isao Tanaka;Y. Ohtsu
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文献类型：
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作者：
M. Yamaguchi;Yasuhiko Kadota;Isao Tanaka;Y. Ohtsu
以载银球形粘土[SC（Ag）]为固定相，对二十二碳六烯酸乙酯（DHA-E）进行了中试精制。因此，通过使用具有高DHA-E含量的鱼油和扩大制备柱，可以在更大程度上回收高纯度的DHA-E（99%）。发现该DHA-E具有试剂级纯度，并且纯化没有伴随劣化。通过将DHA-E的纯度从99%改变为95%，可以缩短纯化所需的时间，并且通过这种新方法可以每天获得更多的更高纯度的DHA-E。
Docosahexaenoic acid ethyl ester (DHA-E) could be refined by a pilot-scale Supercritical Fluid Chromatography (SFC) using silver-loaded spherical clay material [SC (Ag)] in a new chromatographic stationary phase. High purity DHA-E (99%) could thus be recovered to a greater degree by using the fish oil with high DHA-E content, and by enlarging the preparative column. This DHA-E was found to have reagent grade purity, and purification was not attended with deterioration. The time required for purification could be shortened by changing the purity of DHA-E from 99% to 95%, and more DHA-E per day of higher purity could thus be obtained by this new method.