Fine-tuning of pathogenesis-related protein 1 (PR1) activity by the melatonin biosynthetic enzyme ASMT2 in defense response to cassava bacterial blight
Fine-tuning of pathogenesis-related protein 1 (PR1) activity by the melatonin biosynthetic enzyme ASMT2 in defense response to cassava bacterial blight
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DOI：
10.1111/jpi.12784
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发表时间：
2021-12-30
影响因子：
10.3
通讯作者：
Shi, Haitao
中科院分区：
文献类型：
--
作者：
Guo, Jingru;Bai, Yujing;Shi, Haitao
褪黑激素通过调节免疫反应广泛参与植物抗病性。病原相关蛋白在植物免疫应答中起着重要作用。然而，褪黑激素生物合成酶和PR蛋白之间的直接联系仍然是难以捉摸的植物。在这项研究中，我们发现N-乙酰羟色胺O-甲基转移酶2（MeASMT 2）与MePR 1在体外和体内物理相互作用，从而促进MePR 1对Xanthomonas axonopodis pv. Manihotis（Xam）.因此，MeASMT 2增强了MePR 1对木薯抗病性的正调控作用。此外，我们还发现2C型蛋白磷酸酶1（MePP 2C 1）与MeASMT 2相互作用，干扰MePR 1-MeASMT 2相互作用，从而抑制MeASMT 2和MePR 1对木薯抗病性的正向调控作用。与响应于Xam感染的MeASMT 2和MePR 1的转录物增加相反，MePP 2C 1的转录物在Xam感染后减少。因此，疾病激活的MeASMT 2从疾病抑制的MePP 2C 1中释放出来，从而提高了MePR 1的抗菌活性，从而提高了免疫应答。综上所述，本研究阐明了MePP 2C 1-MeASMT 2-MePR 1模块对木薯抗白叶枯病（CBB）防御反应的动态调节，扩展了对褪黑激素生物合成酶与植物PR相关性的理解。
Melatonin is widely involved in plant disease resistance through modulation of immune responses. Pathogenesis-related (PR) proteins play important roles in plant immune responses. However, the direct association between melatonin biosynthetic enzyme and PR protein remains elusive in plants. In this study, we found that N-acetylserotonin O-methyltransferase 2 (MeASMT2) physically interacted with MePR1 in vitro and in vivo, thereby promoting the anti-bacterial activity of MePR1 against Xanthomonas axonopodis pv. manihotis (Xam). Consistently, MeASMT2 improved the effect of MePR1 on positively regulating cassava disease resistance. In addition, we found that type 2C protein phosphatase 1 (MePP2C1) interacted with MeASMT2 to interfere with MePR1-MeASMT2 interaction, so as to inhibiting the effect of MeASMT2 and MePR1 on positively regulating cassava disease resistance. In contrast to the increased transcripts of MeASMT2 and MePR1 in response to Xam infection, the transcript of MePP2C1 was decreased upon Xam infection. Therefore, disease activated MeASMT2 was released from disease inhibited MePP2C1, so as to improving the anti-bacterial activity of MePR1, resulting in improved immune response. In summary, this study illustrates the dynamic modulation of the MePP2C1-MeASMT2-MePR1 module on cassava defense response against cassava bacterial blight (CBB), extending the understanding of the correlation between melatonin biosynthetic enzyme and PR in plants.