Basophilic intracytoplasmic viral matrix inclusions distributed widely in layer hens affected with avian-leukosis-virus-associated tumours
Basophilic intracytoplasmic viral matrix inclusions distributed widely in layer hens affected with avian-leukosis-virus-associated tumours
复制标题
DOI：
10.1080/03079450601110007
复制
发表时间：
2007-02-01
期刊：
影响因子：
2.8
通讯作者：
Yamamoto, Yu
中科院分区：
文献类型：
--
作者：
Nakamura, Kikuyasu;Higahi, Tomoko;Yamamoto, Yu
蛋鸡（3 - 10日龄）与皮下肿瘤的影响进行了病理调查。嗜碱性细胞质内病毒基质包涵体（MI）广泛分布于鸡皮下粘液瘤、横纹肌肉瘤、神经束膜瘤、神经胶质瘤、腹腔腺癌和肾母细胞瘤。在心肌细胞和冲动传导系统细胞中观察到MI。它们还存在于脾和肺动脉的平滑肌细胞中，存在于消化道肌层的平滑肌中（庄稼、食道、前胃、肌胃、十二指肠、空肠、回肠、盲肠和大肠），以及存在于卵巢和胰腺包膜的平滑肌中。它们也被观察到在肾小球足细胞和肾上皮在肾脏，肾母细胞瘤的肿瘤细胞，气管的软骨细胞，鳞状上皮细胞的咽，和神经细胞的大脑和胶质瘤的肿瘤细胞在小脑。组织化学上，MI被RNA染色，但不被DNA染色。各种组织中的MI对禽白血病病毒（ALV）抗原呈强阳性。超微结构上，心肌细胞和肾小球足细胞的胞浆中发现MI。它们由电子致密的小颗粒和环形颗粒组成。在心肌细胞和肾小球足细胞的胞质囊泡中观察到病毒颗粒。用逆转录-聚合酶链反应在肝脏或肿瘤中检测到ALV A亚群特异性基因产物。这一结果表明，MI可以在器官中形成，而不是在自然感染ALV相关肿瘤的鸡的肌肉系统中。
Layer hens (3 10 days old) affected with subcutaneous tumours were investigated pathologically. Basopholic intracytoplasmic viral matrix inclusions (MIs) were widely distributed in the chickens affected with subcutaneous myxoma rhabdomyosarcoma, perineuroma, glioma, intra-abdominal adenocarcinoma, and nephroblastoma. MIs were observed in the myocardial cells and the impulse-conducting-system cells. They were also present in the smooth muscle cells of the arteries in the spleen and lungs, in the smooth muscle of the digestive tract muscular layer (crop, oesophagus, proventriculus, gizzard, duodenum, jejunum, ileum, caecum, and large intestine), and in the smooth muscle of the capsule in the ovary and pancreas. They were also observed in the podocytes of glomeruli and renal epithelium in the kidneys, tumour cells of nephroblastoma, chondrocytes of the trachea, squamous-epithelial cells of the pharynx, and nerve cells of the cerebrum and tumour cells of the glioma in the cerebellum. Histochemically, MIs were stained with RNA, but not with DNA. MIs in the various tissues were strongly positive for the avian leukosis virus (ALV) antigen. Ultrastracturally, MIs were found in the cytoplasm of myocardial cells and podocytes of renal glomeruli. They consisted of electron-dense small granules and ring-shaped particles. Viral particles were observed in the vesicles of the cytoplasm of myocardial cells and glomerular podocytes. The gene product specific for subgroup A of ALV was detected in the livers or tumours by reverse transcription-polymerase chain reaction. This result suggests that MIs can be formed in organs rather than muscular systems in the chickens naturally affected with ALV-associated tumours.