The influence of pH on in vitro hæmolysis in nocturnal hæmoglobinuria
The influence of pH on in vitro hæmolysis in nocturnal hæmoglobinuria
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DOI：
10.1002/path.1700550318
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发表时间：
1943-07-01
期刊：
影响因子：
--
通讯作者：
Richardson, N
中科院分区：
文献类型：
--
作者：
Dacie, JV;Richardson, N
将来自患者去红细胞血液的红细胞洗涤，悬浮在0.9%NaCl中，并加入到血清中，血清的pH值已通过0.9%NaCl中的酸或碱调节。在2种情况下观察到溶血的最佳pH为约7.2，在pH 8以上和pH 6以下具有抑制作用。该pH范围比绵羊或马细胞被人溶血素和补体溶血或正常人细胞被异溶血素和补体溶血时所见的pH范围更受限制，特别是在碱性侧。
Red cells from the patient''s defibrinated blood were washed, suspended in 0.9% NaCl, and added to serum whose pH had been adjusted by acid or alkali in 0.9% NaCl. The optimum pH for hemolysis was observed in 2 cases to be about 7.2, with inhibition above pH 8 and below pH 6. This pH range was more restricted, particularly on the alkaline side, than that seen when sheep or horse cells were hemolysed by human heterolysin and complement or when normal human cells were hemolysed by iso-hemolysin and complement.