A CHEMICAL-MODEL FOR THE COOPERATION OF SULFATES AND CARBOXYLATES IN CALCITE CRYSTAL NUCLEATION - RELEVANCE TO BIOMINERALIZATION
A CHEMICAL-MODEL FOR THE COOPERATION OF SULFATES AND CARBOXYLATES IN CALCITE CRYSTAL NUCLEATION - RELEVANCE TO BIOMINERALIZATION
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DOI：
10.1073/pnas.84.9.2732
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发表时间：
1987-05-01
影响因子：
11.1
通讯作者：
WEINER, S
中科院分区：
文献类型：
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作者：
ADDADI, L;MORADIAN, J;WEINER, S
参与生物晶体生长调节的酸性基质大分子通常含有富含天冬氨酸的结构域和共价结合的硫酸化多糖。我们提出硫酸盐和β-片状结构的羧酸盐在定向方解石晶体成核中协同作用。硫酸盐浓缩钙，产生结构化羧酸盐域上成核所需的过饱和度。由磺化聚苯乙烯表面和吸附的β-片聚天冬氨酸组成的人工模型表明，这两种组分确实在两个独立的测定中协同作用，既通过诱导方解石在(001)平面成核，又通过钙缔合。有证据表明，从软体动物壳中纯化的有机基质酸性糖蛋白在体外可能以类似的方式表现。
Acidic matrix macromolecules involved in regulation of biological crystal growth often contain aspartic acid-rich domains and covalently bound sulfated polysaccharides. We propose that sulfates and .beta.-sheet structured carboxylates cooperate in oriented calcite crystal nucleation. The sulfates concentrate calcium, creating the supersaturation necessary for nucleation on the structured carboxylate domains. An artificial model, composed of sulfonated polystyrene surfaces and adsorbed .beta.-sheet poly(aspartate), demonstrates that the two components indeed act cooperatively with respect to two independent assays, both by induction of calcite nucleation off the (001) plane and by calcium association. Evidence is presented that a purified organic matrix acidic glycoprotein from mollusk shells may behave in vitro in a similar way.