Humoral response and antiviral cytokine expression following vaccination of thoroughbred weanlings-A blinded comparison of commercially available vaccines
Humoral response and antiviral cytokine expression following vaccination of thoroughbred weanlings-A blinded comparison of commercially available vaccines
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DOI：
10.1016/j.vaccine.2013.08.083
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发表时间：
2013-10-25
期刊：
影响因子：
5.5
通讯作者：
Cullinane, Ann
中科院分区：
文献类型：
--
作者：
Gildea, Sarah;Quinlivan, Michelle;Cullinane, Ann
先前在实验马身上使用干扰素-γ作为细胞免疫(CMI)反应的标志物的研究表明，在接种ISCOM亚单位、金丝雀痘重组疫苗和最近的灭活全病毒疫苗后，干扰素-γ基因的表达增加。这项研究的目的是对所有这些疫苗呈递系统接种后引起的体液抗体和CMI反应进行独立的比较。采用单次放射状溶血(SRH)法对44头纯种断奶仔猪进行了二次免疫(V-2)后3周的抗体检测。所有疫苗的抗体反应模式都是相似的。全病毒灭活疫苗(Duvaxyn IE-T Plus)免疫马匹的抗体应答优于ISCOM-基质亚单位疫苗(Equilis PrequenzTe)和金丝雀痘重组疫苗(ProteqFlu-Te)。在这项研究中，39%的断奶仔猪在第一次接种疫苗(V1)后血清转换失败。断奶仔猪接种先锋马和ProteqFlu-Te疫苗后对疫苗(H(3)N8())的反应较差，而接种Duvaxyn IE-T Plus的仔猪对H(3)N8()的反应不明显。分别于VI后第0、2、7、14天采集PAX基因血样，用RT-PCR方法检测干扰素-γ、促炎细胞因子IL-1f3和B细胞刺激因子IL-4的基因表达水平。IL-113和IL-4的平均基因表达水平在免疫后第14天达到峰值。免疫马匹的马匹IL-4基因表达的增加明显大于接种其他两种产品的马匹。免疫后7d，3种疫苗均能显著提高免疫马匹体内干扰素-γ基因的表达，并在免疫后7d达到高峰。总体来看，本研究所用重组灭活疫苗、亚单位疫苗和金丝雀痘重组疫苗免疫马匹后，其干扰素-γ基因的表达没有显著差异。(C)爱思唯尔有限公司出版的2013年。
Previous studies in experimental ponies using interferon gamma (IFN-y) as a marker for cell mediated immune (CMI) response demonstrated an increase in IFN-gamma gene expression following vaccination with an ISCOM subunit, a canarypox recombinant and more recently, an inactivated whole virus vaccine. The objective of this study was to carry out an independent comparison of both humoral antibody and CMI responses elicited following vaccination with all these vaccine presentation systems. Antibody response of 44 Thoroughbred weanlings was monitored for three weeks following the second dose of primary vaccination (V-2) by single radial haemolysis (SRH). The pattern of antibody response was similar for all vaccines. The antibody response of horses vaccinated with the inactivated whole virus vaccine (Duvaxyn IE-T Plus) was superior to that of the horses vaccinated with the ISCOM-matrix subunit (Equilis Prequenza Te) and canarypox recombinant (ProteqFlu-Te) vaccine. In this study 39% of weanlings failed to seroconvert following their first dose of primary vaccination (V1). Poor response to vaccination (H(3)N8()) was observed among weanlings vaccinated with Equilis Prequenza Te and ProteqFlu-Te but not among those vaccinated with Duvaxyn IE-T Plus. PAXgene bloods were collected on days 0, 2, 7 and 14 following VI. Gene expression levels of IFN-y, IL-1f3 (proinflammatory cytokine) and IL-4 (B cell stimulating cytokine) were measured using RT-PCR. Mean gene expression levels of IL-113 and IL-4 peaked on day 14 post vaccination. The increase in IL-4 gene expression by horses vaccinated with Equilis Prequenza Te was significantly greater to those vaccinated with the other two products. Vaccination with all three vaccines resulted in a significant increase in IFN-gamma gene expression which peaked at 7 days post VI. Overall, there was no significant difference in IFN-gamma gene expression by the horses vaccinated with the whole inactivated, the subunit and the canarypox recombinant vaccines included in this study. (C) 2013 Published by Elsevier Ltd.