MODULATION OF POLYAMINE BIOSYNTHESIS AND TRANSPORT BY ONCOGENE TRANSFECTION
MODULATION OF POLYAMINE BIOSYNTHESIS AND TRANSPORT BY ONCOGENE TRANSFECTION
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DOI：
10.1016/s0006-291x(88)80042-1
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发表时间：
1988-11-30
影响因子：
3.1
通讯作者：
PORTER, CW
中科院分区：
文献类型：
--
作者：
CHANG, BK;LIBBY, PR;PORTER, CW
在用EJ 2-大鼠或N-myc癌基因稳定转染的Rat-1细胞中检查癌基因表达对与多胺代谢相关的现象的影响。在ras转染的细胞中，鸟氨酸脱羧酶活性比亲本或N-myc转染的细胞系高约12倍。相反，在N-myc转染的细胞中，多胺摄取显著增加，如它们对多胺类似物N1，N12-双（乙基）精胺（BESm）的抗增殖和酶调节作用的敏感性增强、它们的细胞内BESm积累和它们对甲基甘氨酰双（鸟苷酰腙）的生长抑制作用的敏感性增加所示。N-myc和ras的表达和多胺代谢的关键方面之间的这些关联表明，后者在促进这些癌基因的生长促进特性中可能发挥作用。
The effects of oncogene expression on phenomena related to polyamine metabolism were examined in Rat-1 cells stably transfected with EJ2-rats or N-myc oncogenes. In ras-transfected cells, ornithine decarboxylase activity was about 12-times higher than in either the parent or N-myc-transfected cell lines. By contrast, polyamine uptake was markedly increased in N-myc-transfected cells, as indicated by their enhanced sensitivity to the antiproliferative and enzyme regulatory effects of the polyamine analog, N1, N12-bis(ethyl)spermine (BESm), their intracellular accumulation of BESm and by their increased sensitivity to the growth inhibitory effects of methylglyoxalbis (guanylhydrazone).sbd.another analog which utilizes the polyamine transport mechanism. These associations between N-myc and ras expression and critical aspects of polyamine metabolism suggest a possible role for the latter in facilitating the growth promoting properties of these oncogenes.