Manganese supplementation relieves the phenotypic deficits seen in superoxide-dismutase-null Escherichia coli
Manganese supplementation relieves the phenotypic deficits seen in superoxide-dismutase-null Escherichia coli
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DOI：
10.1016/s0003-9861(02)00065-6
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发表时间：
2002-06-01
影响因子：
3.9
通讯作者：
Benov, L
中科院分区：
文献类型：
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作者：
Al-Maghrebi, M;Fridovich, I;Benov, L
大肠杆菌，缺乏细胞质超氧化物歧化酶，表现出各种氧依赖性表型缺陷。富集的生长培养基与Mn（II）缓解了这些赤字。锰（II）丰富的培养基上生长的细胞提取物表现出超氧化物歧化酶样活性，这是由于部分低分子量和部分高分子量的配合物。高分子量活性对蛋白水解敏感。因此，该活性可能与Mn与蛋白质的低亲和力结合有关。(C)2002 Elsevier Science（美国）。All rights reserved.
Escherichia coli, lacking cytoplasmic superoxide dismutases, exhibits a variety of oxygen-dependent phenotypic deficits. Enrichment of the growth medium with Mn(II) relieved those deficits. Extracts of cells grown on Mn(II)-rich medium exhibited superoxide dismutase-like activity that was due partially to low-molecular-weight and partially to high-molecular-weight complexes. The high-molecular-weight activity was sensitive to proteolysis. Hence this activity is likely associated with low-affinity binding of Mn to proteins. (C) 2002 Elsevier Science (USA). All rights reserved.