Using saturated absorption for superresolution laser scanning transmission microscopy
Using saturated absorption for superresolution laser scanning transmission microscopy
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DOI：
10.1111/jmi.13033
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发表时间：
2021-06-08
影响因子：
2
通讯作者：
Fujita, Katsumasa
中科院分区：
文献类型：
--
作者：
Nishida, Kentaro;Sato, Hikaru;Fujita, Katsumasa
我们通过利用染料分子的饱和吸收提高了激光扫描透射显微镜的三维空间分辨率。饱和吸收是由高强度光照射引起的，并将信号定位在焦点的中心内。我们的数值计算表明，使用饱和吸收引起的非线性透射信号，透射成像的空间分辨率在横向和轴向上均得到显着提高。我们通过观察染色的大鼠肾组织的精细结构，实验证明了三维分辨率的提高，这是传统激光扫描透射显微镜无法观察到的。
We improved the three-dimensional spatial resolution of laser scanning transmission microscopy by exploiting the saturated absorption of dye molecules. The saturated absorption is induced by the high-intensity light irradiation and localises the signal within the centre of the focal spot. Our numerical calculation indicates that the spatial resolution in transmission imaging is significantly improved for both lateral and axial directions using nonlinear transmitted signals induced by saturated absorption. We experimentally demonstrated the improvement of the three-dimensional resolution by observing fine structures of stained rat kidney tissues, which were not able to be visualised by conventional laser scanning transmission microscopy.