Transforming activity of human papillomavirus type 16 DNA sequence in a cervical cancer.
Transforming activity of human papillomavirus type 16 DNA sequence in a cervical cancer.
复制标题
人乳头瘤病毒 16 型 DNA 序列在宫颈癌中的转化活性。
DOI：
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发表时间：
1986
影响因子：
11.1
通讯作者：
Takashi Sugimura
中科院分区：
文献类型：
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作者：
Y. Tsunokawa;N. Takebe;Tatsuhiro KASAMATSUt;M. Terada;Takashi Sugimura
使用磷酸钙共沉淀技术进行转染试验时，发现从宫颈癌组织中提取的含有人乳头瘤病毒（HPV） 16型的基因组DNA样本可诱导NIH 3T3细胞恶性转化。初级和次级转化子包含HPV 16型DNA序列和人类特异性Alu家族序列。据我们所知，以前没有报道过宫颈癌总基因组DNA中HPV 16型DNA序列具有转化活性。
A genomic DNA sample from cervical cancer tissue, containing human papillomavirus (HPV) type 16, was found to induce malignant transformation of NIH 3T3 cells when it was tested by transfection assays using the calcium phosphate coprecipitation technique. The primary and secondary transformants contained the HPV type 16 DNA sequences and human specific Alu family sequences. To the best of our knowledge, it has not been reported previously that HPV type 16 DNA sequences in total genomic DNA from a cervical cancer have transforming activity.