Expression of (pro)renin receptor in human kidneys with end-stage kidney disease due to diabetic nephropathy
Expression of (pro)renin receptor in human kidneys with end-stage kidney disease due to diabetic nephropathy
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DOI：
10.1016/j.peptides.2010.04.003
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发表时间：
2010-07-01
期刊：
影响因子：
3
通讯作者：
Totsune, Kazuhito
中科院分区：
文献类型：
--
作者：
Takahashi, Kazuhiro;Yamamoto, Hajime;Totsune, Kazuhito
（Pro）renin receptor（（P）RR）是一种由350个氨基酸组成的具有单一跨膜结构域的蛋白质，是肾素和原肾素的特异性受体，在糖尿病肾病中可能发挥重要的病理生理作用。本研究的目的是阐明（P）RR在糖尿病肾病终末期肾病肾脏中的表达。尸检时从患有和不患有2型糖尿病的患者（n = 5，不患有糖尿病; n=8，患有糖尿病）中获得肾脏组织。免疫细胞化学显示（P）RR主要表达于非糖尿病肾病肾的肾小管细胞和集合管细胞。肾小球细胞中的（P）RR免疫染色非常弱且零星。血管平滑肌细胞和内皮细胞对RR的免疫染色非常弱或不染色。肾周脂肪组织中的脂肪细胞呈（P）RR免疫染色阳性。（P）RR在糖尿病肾病组的免疫组化染色模式与无糖尿病肾病组相似。然而，最值得注意的是，在患有糖尿病肾病直至终末期肾病的肾脏中，在肾小管细胞和集合管细胞中清楚且经常更强烈地观察到（P）RR免疫染色。本研究提出了糖尿病肾脏中表达的（P）RR可能在终末期肾病中发现的血管紧张素I生成和肾纤维化中发挥病理生理作用的可能性。(C)2010年爱思唯尔公司All rights reserved.
(Pro)renin receptor ((P)RR), a specific receptor for renin and prorenin, is a 350 amino-acid protein with a single transmembrane domain and may play important pathophysiological roles in diabetic nephropathy. The aim of the present study is to clarify the expression of (P)RR in the kidney with end-stage renal disease due to diabetic nephropathy. The kidney tissues were obtained at autopsy from patients with and without Type 2 diabetes mellitus (n = 5 without diabetes mellitus; and n=8 with diabetes mellitus). Immunocytochemistry showed that (P)RR was mainly expressed in the tubular cells and collecting duct cells of the kidney without diabetic nephropathy. Cells in glomeruli were very weakly and sporadically immunostained for (P)RR. Vascular smooth muscle cells and endothelial cells were very weakly or were not immunostained for (P)RR. Adipocytes in the adipose tissue around the kidney were positively immunostained for (P)RR. lmmunostaining pattern of (P)RR in the kidney with diabetic nephropathy was similar to that without diabetic nephropathy. However, most notably, (P)RR immunostaining in the tubular cells and collecting duct cells was clearly and frequently more strongly observed in the kidney with diabetic nephropathy up to the end-stage renal disease. The present study has raised the possibility that (P)RR expressed in the diabetic kidney may play a pathophysiological role in angiotensin I generation and renal fibrosis found in end-stage renal disease. (C) 2010 Elsevier Inc. All rights reserved.