ANALYSIS OF MIXED INFECTION OF SHEEP WITH BLUETONGUE VIRUS SEROTYPE-10 AND SEROTYPE-17 - EVIDENCE FOR GENETIC REASSORTMENT IN THE VERTEBRATE HOST
ANALYSIS OF MIXED INFECTION OF SHEEP WITH BLUETONGUE VIRUS SEROTYPE-10 AND SEROTYPE-17 - EVIDENCE FOR GENETIC REASSORTMENT IN THE VERTEBRATE HOST
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DOI：
10.1128/jvi.61.4.1086-1091.1987
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发表时间：
1987-04-01
影响因子：
5.4
通讯作者：
RAMIG, RF
中科院分区：
文献类型：
--
作者：
SAMAL, SK;LIVINGSTON, CW;RAMIG, RF
用蓝舌病病毒（BTV）血清型10和BTV血清型17各109 PFU静脉内感染两只血清阴性绵羊。一只动物出现轻度蓝舌病样疾病，两只动物均出现短期病毒血症，并对两种病毒血清型产生中和免疫应答。通过使用分离期间不会发生重配的条件，从每只动物的静脉血中分离子代病毒。确定了感染绵羊的子代病毒的电泳型，这两种动物的结果惊人地相似。在这两个绵羊，血清型10占主导地位的后代，占92%的后代。血清型17很少分离，占分析后代的3%。剩余的5%的子代克隆是来自血清型10和17的抗性和衍生的基因组区段。对少数抗性后代中基因组片段的亲本来源进行分析表明，感染绵羊中可能发生了特定基因组片段的选择。这些数据表明，基因组片段的重排发生，在低频率，在绵羊混合感染BTV。
Two seronegative sheep were infected intravenously with 109 PFU each of bluetongue virus (BTV) serotype 10 and BTV serotype 17. One animal experienced a mild bluetongue-like disease, and both experienced a short-duration viremia and developed neutralizing immune responses to both virus serotypes. Progeny virus was isolated from venous blood from each animal by using conditions in which reassortment could not have occurred during isolation. Electropherotypes were determined for the progeny viruses from the infected sheep, yielding strikingly similar results for the two animals. In both sheep, serotype 10 dominated among the progeny, accounting for 92% of the progeny. Serotype 17 was rarely isoalted and accounted for 3% of the progeny analyzed. The remaining 5% of the progeny clones were reassortant and derived genome segments from both serotypes 10 and 17. Analysis of the parental origin of genome segments in the small number of reassortant progeny analyzed suggested that selection of specific genome segments may have occurred in the infected sheep. These data indicate that reassortment of genome segments occurs, at low frequency, in sheep mixedly infected with BTV.