Dependence on protein synthesis of the N6-monobutyryl cyclic AMP plus theophylline mediated release of luteinizing hormone induced by luteinizing hormone releasing hormone from rat pituitary glands in vitro.
Dependence on protein synthesis of the N6-monobutyryl cyclic AMP plus theophylline mediated release of luteinizing hormone induced by luteinizing hormone releasing hormone from rat pituitary glands in vitro.
复制标题
体外大鼠垂体促黄体生成素释放激素诱导的黄体生成素释放对 N6-单丁酰环 AMP 加茶碱介导的蛋白质合成的依赖性。
DOI：
10.1677/joe.0.0880329
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
G. van Rees
中科院分区：
文献类型：
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作者：
J. D. de Koning;J. van Dieten;A. Tijssen;G. van Rees
通过在发情间第 2 天将成年雌性大鼠的垂体与 1 mN-N6-单丁酰环 AMP (mbcAMP) 和 10 mM-茶碱一起孵育长达 10 小时，研究了环 AMP 参与 LH 释放激素 (LH-RH) 对 LH 分泌的作用。这种处理诱导的 LH 释放模式类似于在低浓度 LH-RH（0.1 ng LH-RH/ml）存在下观察到的模式，即最初 4 小时期间 LH 释放最少，随后释放速率增加。在该系统中，放线菌酮（25微克/毫升）对蛋白质合成的抑制不会损害垂体组织的初始反应，但在反应的第二阶段期间LH释放速率的增加被阻止。与 mbcAMP 和茶碱预孵育可增加垂体组织对 LH-RH 的反应性。通过在预孵育期间添加放线菌酮可以防止这种作用，而在与 LH-RH 一起孵育期间添加该药物不再损害增加的反应性。通过诱导蛋白质合成介导的mbcAMP和茶碱的敏化作用的大小与高浓度的LH-RH相当。由于预孵育期间总 LH 没有显着变化，因此可以得出结论，反应性的增加不是新合成的 LH 的结果。目前的结果表明环AMP在LH-RH诱导的LH分泌中的一个或多个作用。除了对与蛋白质合成相关的因子的形成产生影响之外，除了LH在LH的急性释放中起许可作用之外，环AMP还可能通过不涉及蛋白质合成的途径参与分泌过程。
The involvement of cyclic AMP in the action of LH releasing hormone (LH-RH) on LH secretion was studied by incubating pituitary glands from adult female rats on day 2 of dioestrus with 1 mN-N6-monobutyryl cyclic AMP (mbcAMP) and 10 mM-theophylline for periods of up to 10 h. This treatment induced a pattern of LH release similar to that observed in the presence of a low concentration of LH-RH (0.1 ng LH-RH/ml), i.e. an initial 4 h period during which the release of LH was minimal was followed subsequently by an increased rate of release. In this system inhibition of protein synthesis by cycloheximide (25 microgram/ml) did not impair the initial response of the pituitary tissue but the increase in the rate of LH release during the second phase of the response was blocked. Preincubation with mbcAMP and theophylline increased the responsiveness of the pituitary tissue to LH-RH. This action could be prevented by including cycloheximide during the preincubation period, whereas addition of this drug during the incubation with LH-RH no longer impaired the increased responsiveness. The size of the sensitizing action of mbcAMP and theophylline mediated through the induction of protein synthesis was comparable with that of a high concentration of LH-RH. From the absence of a significant change in total LH during the preincubation period, it was concluded that the increased responsiveness was not the result of newly synthesized LH. The present results suggest a role or roles for cyclic AMP in the secretion of LH induced by LH-RH. Besides an effect on the formation of a factor related to the synthesis of protein, other than LH which has a permissive role in the acute release of LH, cyclic AMP might also be concerned in the secretion process through a pathway which does not involve synthesis of protein.