High-frequency oscillations after status epilepticus: Epileptogenesis and seizure genesis
High-frequency oscillations after status epilepticus: Epileptogenesis and seizure genesis
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DOI：
10.1111/j.0013-9580.2004.17004.x
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发表时间：
2004-09-01
期刊：
影响因子：
5.6
通讯作者：
Engel, T
中科院分区：
文献类型：
--
作者：
Bragin, A;Wilson, CL;Engel, T
目的：目的：探讨海马内海人藻酸盐诱发癫痫持续状态后，海马齿状回高频振荡（HFOs）与反复自发性癫痫发作的时间关系。在大鼠海马CA 3区右后上方植入海人酸（KA）注射导管。记录基线电活动后，注射KA（0.4 μ g/0.2穆尔）。从第二天开始，记录电图活动与视频监控癫痫发作，每天8小时/天大于或等于30 day.Results：26只大鼠研究，19揭示了出现的尖波活动和HFO的频率范围为80至500 Hz的同侧的齿状回KA注射。在其余7只大鼠中，在该频率范围内未观察到明显的活动。在一些反复发作的大鼠中，HFO在涟漪频率范围内（100-200 Hz）;在其他大鼠中，HFO在快速涟漪频率范围内（200-500 Hz），或发现两种振荡频率的混合物。癫痫持续状态后首次检测到HFO的时间在1至30天之间变化，平均值为6.3 ± 2.0（SEM）。在出现HFO活动的19只大鼠中，所有大鼠后来都发生了复发性自发性癫痫发作，而没有HFO的大鼠都没有发生癫痫发作。癫痫持续状态后越早检测到HFO活性，首次自发性癫痫发作发生越早。一个显着的负相关关系被发现之间的时间到第一HFO检测和随后的自发性seizure.Conclusions率：一个很强的相关性被发现之间的时间减少检测HFOs和自发性癫痫发作率的增加，以及减少KA注射和自发性癫痫发作的检测之间的潜伏期的持续时间。KA注射后发现两种类型的HFO，一种在100至200 Hz的频率范围内，另一种在200至500 Hz的频率范围内，两者都应被认为是病理性的，这表明两者都是致癫痫的。
Purpose: To investigate the temporal relation between high-frequency oscillations (HFOs) in the dentate gyrus and recurrent spontaneous seizures after intrahippocampal kainite-induced status epilepticus.Methods: Recording microelectrodes were implanted bilaterally in different regions of hippocampus and entorhinal cortex. A guide cannula for microinjection of kainic acid (KA) was implanted above the right posterior CA3 area of hippocampus. After recording baseline electrical activity, KA (0.4 mug/0.2 mul) was injected. Beginning on the next day, electrographic activity was recorded with video monitoring for seizures every day for 8 h/day for greater than or equal to30 days.Results: Of the 26 rats studied, 19 revealed the appearance of sharp-wave activity and HFOs in the frequency range of 80 to 500 Hz in the dentate gyrus ipsilateral to the KA injection. In the remaining seven rats, no appreciable activity was noted in this frequency range. In some rats with recurrent seizures, HFOs were in the ripple frequency range (100-200 Hz); in others, HFOs were in the fast ripple frequency range (200-500 Hz), or a mixture of both oscillation frequencies was found. The time of detection of the first HFOs after status epilepticus varied between 1 and 30 days, with a mean of 6.3 +/- 2.0 (SEM). Of the 19 rats in which HFO activity appeared, all later developed recurrent spontaneous seizures, whereas none of the rats without HFOs developed seizures. The sooner HFO activity was detected after status epilepticus, the sooner the first spontaneous seizure occurred. A significant inverse relation was found between the time to the first HFO detection and the subsequent rate of spontaneous seizures.Conclusions: A strong correlation was found between a decreased time to detection of HFOs and an increased rate of spontaneous seizures, as well as with a decrease in the duration of the latent period between KA injection and the detection of spontaneous seizures. Two types of HFOs were found after KA injection, one in the frequency range of 100 to 200 Hz, and the other, in the frequency range of 200 to 500 Hz, and both should be considered pathological, suggesting that both are epileptogenic.