MutScreener: primer design tool for PCR-direct sequencing
MutScreener: primer design tool for PCR-direct sequencing
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DOI：
10.1093/nar/gkl168
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发表时间：
2006-07-01
影响因子：
14.9
通讯作者：
Liu, Chunyu
中科院分区：
文献类型：
--
作者：
Yao, Fengxia;Zhang, Ruifang;Liu, Chunyu
在寻找易感基因方面，定位克隆和候选基因策略都很有帮助。突变筛查是为了识别候选基因或基因组区域的突变或多态而实施的众多技术之一。由于人类基因组序列是已知的，所以聚合酶链式反应直接测序是进行突变筛查或重测序的主要方法之一。不幸的是，如果需要研究多个基因或大区域，分析设计可能会很费力。为了解决这一难题，已经开发了一个基于Web的应用程序MutScreener。MutScreener协助分析人类基因结构和设计聚合酶链式反应/测序引物。此应用程序支持基于现有公共基因注释或定制基因注释的批量分析设计。可选的通用标记引物可以支持高通量的重测序过程。MutScreener可在http://bioinfo.bsd.uchicago.edu/MutScreener.html.上供公众使用
In searching for susceptibility genes, both positional cloning and candidate gene strategies have been helpful. Mutation screening is one of the many technologies that have been implemented in order to identify mutations or polymorphisms in candidate genes or genomic regions. Since human genome sequence is available, PCR- direct sequencing is one of the major methods for mutation screening or resequencing. Unfortunately, assay design can be laborious if multiple genes or large regions need to be investigated. To solve this conundrum a web-based application, MutScreener, has been developed. MutScreener assists in the analysis of human gene structure and design of PCR/ sequencing primer. This application supports batch assay design based on either existing public gene annotation or custom gene annotation. The optional universal tagged primers can support high throughput resequencing processes. MutScreener is available for public use at http://bioinfo.bsd.uchicago.edu/MutScreener.html.