Spontaneous membrane fusion induced by chemical formation of ceramides in a lipid bilayer
Spontaneous membrane fusion induced by chemical formation of ceramides in a lipid bilayer
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DOI：
10.1021/ja0652969
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发表时间：
2006-11-15
影响因子：
15
通讯作者：
Tani, Shohei
中科院分区：
文献类型：
--
作者：
Kunishima, Munetaka;Tokaji, Masafumi;Tani, Shohei
在小单层囊泡（SUV）的膜中神经酰胺和相关双链脂质的单纯化学生成诱导囊泡的融合。通过使用2-氯-4，6-二甲氧基-1，3，5-三嗪和两亲性叔胺催化剂的组合，可以通过SUV的脂质双层中单链脂质（脂肪酸和鞘氨醇或其类似物）之间的脱氢缩合成功地制备脂质，该方法可以与脂肪酰基-CoA合成酶随后酰基转移酶的连续酶模型系统进行比较。SUV在人工酶系统的这种内部化学刺激下自发地进行膜融合。
Mere chemical generation of ceramide and related double-chain lipids in the membrane of small unilamellar vesicles (SUVs) induces fusion of the vesicles. The lipids can be successfully prepared by dehydrocondensation between single-chain lipids (fatty acids and sphingosine or its analogues) in a lipid bilayer of the SUV by using a combination of 2-chloro-4,6-dimethoxy-1,3,5-triazine and amphiphilic tertiary amine catalysts, a process that can be compared to a successive enzyme model system for a fatty acyl-CoA synthetase followed by acyltransferase. The SUV spontaneously undergoes membrane fusion upon this internal chemical stimulation by the artificial enzyme system.