Determinants of cytoplasmic microtubule depolymerization during ciliogenesis in Chlamydomonas.
Determinants of cytoplasmic microtubule depolymerization during ciliogenesis in Chlamydomonas.
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DOI：
10.26508/lsa.202302287
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发表时间：
2024-01
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
--
作者：
先前显示化学诱导的脱纤毛可诱导细胞质微管解聚，其发生与纤毛损失无关。此外，在聚合细胞质微管存在的情况下可以立即发生纤毛发生。纤毛的核心是微管，它确定长度并协助纤毛组装和拆卸。然而，纤毛外部的微管可以调节纤毛发生。微管细胞骨架快速聚合和解聚。这些过程已经在具有化学和遗传扰动的各种生物体中进行了研究。然而，这些研究在纤毛发生过程中细胞质微管（CytoMT）和游离微管蛋白动力学的作用方面产生了相互矛盾的数据。在这里，我们利用化学和机械扰动来研究莱茵衣藻纤毛发生和 CytoMT 动力学之间的关系。我们发现，稳定的 CytoMT 不仅可以实现正常的纤毛组装，而且高钙浓度和低 pH 诱导的纤毛作用会导致 CytoMT 与纤毛脱落分开解聚。此外，尽管 CytoMT 完整，但通过机械剪切的纤毛脱落使纤毛能够更早再生。我们的数据表明，CytoMT 不是衣藻细胞质微管蛋白限制库的接收器，脱纤毛后的解聚是纤毛发生的结果而不是必需的，并且细胞质和近端纤毛中的完整微管蛋白支持更有效的纤毛组装。
Chemically induced deciliation previously shown to induce cytoplasmic microtubule depolymerization occurs independently of ciliary loss. Furthermore, immediate ciliogenesis can occur in the presence of polymerized cytoplasmic microtubules. At the core of cilia are microtubules which establish length and assist ciliary assembly and disassembly; however, microtubules outside of the cilium can regulate ciliogenesis. The microtubule cytoskeleton polymerizes and depolymerizes rapidly. These processes have been studied across various organisms with chemical and genetic perturbations. However, these have generated conflicting data in terms of the role of cytoplasmic microtubules (CytoMTs) and free tubulin dynamics during ciliogenesis. Here, we look at the relationship between ciliogenesis and CytoMT dynamics in Chlamydomonas reinhardtii using chemical and mechanical perturbations. We find that not only can stabilized CytoMTs allow for normal ciliary assembly, but high calcium concentrations and low pH-induced deciliation cause CytoMTs to depolymerize separately from ciliary shedding. In addition, ciliary shedding through mechanical shearing allows cilia to regenerate earlier despite intact CytoMTs. Our data suggest that CytoMTs are not a sink for a limiting pool of cytoplasmic tubulin in Chlamydomonas, depolymerization after deciliation is a consequence rather than a requirement for ciliogenesis, and intact tubulin in the cytoplasm and proximal cilium support more efficient ciliary assembly.
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影响因子：
5.3
作者：
STARGELL, LA;HERUTH, DP;GOROVSKY, MA
通讯作者：
GOROVSKY, MA
影响因子：
3.3
作者：
Sharma N;Kosan ZA;Stallworth JE;Berbari NF;Yoder BK
通讯作者：
Yoder BK
DOI：
10.1083/jcb.91.2.479
发表时间：
1981-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Parness J;Horwitz SB
通讯作者：
Horwitz SB
微管及其在癌症细胞应激中的作用。
影响因子：
4.7
作者：
Parker AL;Kavallaris M;McCarroll JA
通讯作者：
McCarroll JA
高分辨率微管结构揭示了 GTP 水解后 αβ-微管蛋白的结构转变。
影响因子：
64.5
作者：
Alushin GM;Lander GC;Kellogg EH;Zhang R;Baker D;Nogales E
通讯作者：
Nogales E