rdgB knockdown in neurons reduced nocturnal sleep in Drosophila melanogaster
rdgB knockdown in neurons reduced nocturnal sleep in Drosophila melanogaster
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DOI：
10.1016/j.bbrc.2022.12.043
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发表时间：
2022-12-29
影响因子：
3.1
通讯作者：
Kume, Kazuhiko
中科院分区：
文献类型：
--
作者：
Kobayashi, Riho;Yamashita, Yuko;Kume, Kazuhiko
最近的研究表明，行为定义的睡眠在从昆虫到人类的广泛物种中都是保守的。对于进化分析，确定同源基因如何调节物种间的同源功能是至关重要的。果蝇与哺乳动物有许多与睡眠相关的基因，包括SIK3，即盐诱导蛋白3，它的突变导致小鼠和果蝇长时间睡眠。果蝇rdgB(视网膜变性B)编码一种膜相关的磷脂酰肌醇转移蛋白，它的突变导致光感受器细胞的退化。RdgB突变也损害了光传导和嗅觉行为，表明rdgB参与了正常的神经传递。哺乳动物rdgB同源物Pitpnm2(磷脂酰肌醇转移蛋白膜相关蛋白2)被发现是睡眠需要指数磷蛋白(SNIPPs)之一，提示其在睡眠中的作用。在这里，我们证明了rdgB参与了果蝇的睡眠调节。泛神经元和蘑菇体(MB)特异性rdgB基因敲除减少了夜间睡眠。MB神经元占主导地位，rdgB仅在泛神经元敲除的MB神经元中表达，逆转了rdgB敲除的睡眠减少效应。这些结果揭示了rdgB在果蝇中与睡眠相关的功能，这可能是跨物种保存的。(C)2022 Elsevier Inc.保留所有权利。
Recent studies revealed behaviorally defined sleep is conserved across broad species from insect to human. For evolutional analysis, it is critical to determine how homologous genes regulate the homol-ogous function among species. Drosophila melanogaster shares numerous sleep related genes with mammals including Sik3, salt-inducible kinase 3, whose mutation caused long sleep both in mouse and fruit fly. The Drosophila rdgB (retinal degeneration B) encodes a membrane-associated phosphatidyli-nositol transfer protein and its mutation caused light-induced degeneration of photoreceptor cells. rdgB mutation also impaired phototransduction and olfactory behavior, indicating rdgB is involved in the normal neural transmission. Mammalian rdgB homologue, Pitpnm2 (phosphatidylinositol transfer pro-tein membrane-associated 2) was discovered as one of SNIPPs (sleep-need index phosphoproteins), suggesting its role in sleep. Here, we show that rdgB is involved in sleep regulation in Drosophila. Pan -neuronal and mushroom body (MB) specific rdgB knockdown decreased nocturnal sleep. MB neurons play a dominant role, since the rescue of rdgB expression only in MB neurons in pan-neuronal knock-down reversed the sleep reducing effect of rdgB knockdown. These results revealed the sleep-related function of rdgB in Drosophila which may be conserved across species.(c) 2022 Elsevier Inc. All rights reserved.