A Polymer/Peptide Complex-Based Sensor Array That Discriminates Bacteria in Urine
A Polymer/Peptide Complex-Based Sensor Array That Discriminates Bacteria in Urine
复制标题
DOI：
10.1002/anie.201706101
复制
发表时间：
2017-11-27
影响因子：
16.6
通讯作者：
Bunz, Uwe H. F.
中科院分区：
文献类型：
--
作者：
Han, Jinsong;Cheng, Haoran;Bunz, Uwe H. F.
带负电荷的聚对苯撑乙炔（PPE）与四种带正电荷的抗菌肽（AMP）形成静电复合物。AMP部分淬灭PPE的荧光，并通过基于模式的荧光识别来区分水中和人尿液中的14种不同细菌; AMP-PPE复合物与细菌表面的组分有差异地结合。根据染色特性（革兰氏阳性和革兰氏阴性）或遗传相似性（属、种和菌株），细菌种和菌株形成簇。识别和数据处理是通过模式评估与线性判别分析（LDA）收集的荧光强度数据。
A negatively charged poly(para-phenyleneethynylene) (PPE) forms electrostatic complexes with four positively charged antimicrobial peptides (AMP). The AMPs partially quench the fluorescence of the PPE and discriminate fourteen different bacteria in water and in human urine by pattern-based fluorescence recognition; the AMP-PPE complexes bind differentially to the components of bacterial surfaces. The bacterial species and strains form clusters according to staining properties (Gram-positive and Gram-negative) or genetic similarity (genus, species, and strain). The identification and data treatment is performed by pattern evaluation with linear discriminant analysis (LDA) of the collected fluorescence intensity data.