Mosaic genealogy of the Mus musculus genome revealed by 21 nuclear genes from its three subspecies.
Mosaic genealogy of the Mus musculus genome revealed by 21 nuclear genes from its three subspecies.
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DOI：
10.1266/ggs.83.77
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发表时间：
2008-02
影响因子：
1.1
通讯作者：
Yu-Hua Liu;Aya Takahashi;T. Kitano;Tsuyoshi Koide;T. Shiroishi;K. Moriwaki;N. Saitou
中科院分区：
文献类型：
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作者：
Yu-Hua Liu;Aya Takahashi;T. Kitano;Tsuyoshi Koide;T. Shiroishi;K. Moriwaki;N. Saitou
遗传变异的模式提供了对物种进化历史的深入了解。小鼠（Mus musculus）是实现此目的的一个很好的模型。在这里，我们基于来自三个小家鼠亚种（小家鼠、家养和栗）的九个近交菌株和作为外群的一种小家鼠菌株的核苷酸序列，对小家鼠的 21 个核位点和一个线粒体 DNA 区域进行了谱系分析。这些品系的线粒体 DNA 基因谱系证实了一种肌肉品系的基因渗入模式。当所有核DNA数据连接起来产生九个菌株的系统发育树时，musculus和domesticus菌株彼此形成单系簇，而两个castaneus菌株是并系的。当每个DNA区域被独立处理时，系统发育网络揭示了不可忽视的高水平的亚种混合及其基因组的镶嵌性质。对祖先和衍生种群规模以及迁移率的估计表明祖先多态性和基因流对当前亚种遗传变异模式的影响。 Fut4 和 Dfy 位点的基因谱系也表明小家鼠和毛鼠或其他远缘物种之间存在基因流。
Patterns of genetic variation provide insight into the evolutionary history of a species. Mouse (Mus musculus) is a good model for this purpose. Here we present the analysis of genealogies of the 21 nuclear loci and one mitochondrial DNA region in M. musculus based on our nucleotide sequences of nine inbred strains from three M. musculus subspecies (musculus, domesticus, and castaneus) and one M. spicilegus strain as an outgroup. The mitochondrial DNA gene genealogy of those strains confirmed the introgression pattern of one musculus strain. When all the nuclear DNA data were concatenated to produce a phylogenetic tree of nine strains, musculus and domesticus strains formed monophyletic clusters with each other, while the two castaneus strains were paraphyletic. When each DNA region was treated independently, the phylogenetic networks revealed an unnegligibly high level of subspecies admixture and the mosaic nature of their genome. Estimation of ancestral and derived population sizes and migration rates suggests the effects of ancestral polymorphism and gene flow on the pattern of genetic variation of the current subspecies. Gene genealogies of Fut4 and Dfy loci also suggested existence of the gene flow between M. musculus and M. spicilegus or other distant species.