K+-induced swelling of the dogfish shark (Squalus acanthias) rectal gland cells is associated with changes of the cytoskeleton.
K+-induced swelling of the dogfish shark (Squalus acanthias) rectal gland cells is associated with changes of the cytoskeleton.
复制标题
K 引起的角鲨（Squalus acanthias）直肠腺细胞肿胀与细胞骨架的变化有关。
DOI：
10.1016/0167-4889(89)90238-3
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Mills,JW
中科院分区：
文献类型：
--
作者：
Kleinzeller,A;Mills,JW
角鲨（Squalus acanthias）的直肠腺细胞在等量的K+取代Na+的弹性分支培养基中大量膨胀；当过量的葡萄糖酸盐取代Cl−时，这种肿胀被消除，而细胞去极化在两种培养基中是相似的。K+效应与(a)稳态42k（和86rb）流出（特别是快速细胞流出成分的速率常数）的增加和K+各自细胞池的重新排列有关；(b)细胞形态的改变和f -肌动蛋白沿基底外侧细胞膜的染色模式，这是用阳具酸素的荧光类似物显示的。这些变化与细胞体积无关，在KCl和k -葡萄糖酸盐培养基中相同。观察结果是针对K+（和Rb+）的：用Li+（不被细胞积极挤出）或瓦巴因的存在替代介质Na+，只产生轻微的肿胀，而不影响细胞形态和f作用分布。结果与以下观点一致：与Na+或Li+介质相反，K+诱导的细胞骨架皮层f -肌动蛋白成分的变化允许由于细胞内不均匀阴离子的渗透作用而观察到的大量细胞肿胀。
Dogfish shark (Squalus acanthias) rectal gland cells swell massively when incubated in elasmobranch media in which Na+was equivalently replaced by K+; this swelling was abolished when the impermeant gluconate replaced Cl−, while the cell depolarization was comparable in both media. The K+-effect was associated with (a) an increase of the steady-state42K (and86Rb) efflux (particularly of the rate constant of the fast cellular efflux component) and a rearrangement of the respective cellular pools of K+; (b) an alteration of cell morphology and the pattern of the F-actin staining along the basolateral cell membrane as revealed with fluorescent analogs of phallacidin. These changes were independent of cell volume, being identical in KCl and K-gluconate media. The observations were specific for K+(and Rb+): replacement of media Na+by Li+(which is not actively extruded by the cells), or the presence of ouabain, produced only minor swelling without affecting cell morphology and F-acting distribution. The results are consistent with the following view: as opposed to Na+or Li+media, the K+-induced changes of the cortical F-actin component of the cytoskeleton permit the observed massive cell swelling due to the osmotic contribution of intracellular impermeant anion(s).