Time-lapse imaging of disease progression in deep brain areas using fluorescence microendoscopy.
Time-lapse imaging of disease progression in deep brain areas using fluorescence microendoscopy.
复制标题
作者：
活体显微镜和疾病动物模型的结合推动了疾病机制和治疗方法的研究。然而，许多疾病折磨组织无法在活体光学显微镜。在这里，我们介绍了细胞水平的延时成像深入活哺乳动物大脑内的单光子和双光子荧光显微内窥镜在多个星期。双侧成像部位允许对个体受试者进行纵向比较，包括正常和病变组织。使用这种方法，我们追踪了成年小鼠的CA 1海马锥体神经元树突，揭示了这些树突的极端稳定性（>8,000天的平均寿命）和罕见的结构改变。为了说明疾病研究，我们通过观察肿瘤生长，可视化三维血管结构和确定微循环速度来跟踪深部胶质瘤。尽管毛细血管直径显著增加，但随着疾病的进展，胶质瘤的平均红细胞速度显著下降。延时显微内窥镜将适用于许多疾病的研究，包括神经血管，神经，癌症和创伤引起的条件。
The combination of intravital microscopy and animal models of disease has propelled studies of disease mechanisms and treatments. However, many disorders afflict tissues inaccessible to light microscopy in live subjects. Here we introduce cellular-level time-lapse imaging deep within the live mammalian brain by one- and two-photon fluorescence microendoscopy over multiple weeks. Bilateral imaging sites allowed longitudinal comparisons within individual subjects, including of normal and diseased tissues. Using this approach we tracked CA1 hippocampal pyramidal neuron dendrites in adult mice, revealing these dendrites' extreme stability (>8,000 day mean lifetime) and rare examples of their structural alterations. To illustrate disease studies, we tracked deep lying gliomas by observing tumor growth, visualizing three-dimensional vasculature structure, and determining microcirculatory speeds. Average erythrocyte speeds in gliomas declined markedly as the disease advanced, notwithstanding significant increases in capillary diameters. Time-lapse microendoscopy will be applicable to studies of numerous disorders, including neurovascular, neurological, cancerous, and trauma-induced conditions.
登录
查看更多内容
影响因子：
25
作者：
Chow, David K.;Groszer, Matthias;Pribadi, Mochtar;Machniki, Michal;Carmichael, S. Thomas;Liu, Xin;Trachtenberg, Joshua T.
通讯作者：
Trachtenberg, Joshua T.
影响因子：
64.8
作者：
Garkavtsev, I;Kozin, SV;Jain, RK
通讯作者：
Jain, RK
影响因子：
5.3
作者：
Li, Ping;Murphy, Timothy H.
通讯作者：
Murphy, Timothy H.
影响因子：
64.8
作者：
Meyer-Luehmann, Melanie;Spires-Jones, Tara L.;Hyman, Bradley T.
通讯作者：
Hyman, Bradley T.
影响因子：
9.8
作者：
Lee WC;Huang H;Feng G;Sanes JR;Brown EN;So PT;Nedivi E
通讯作者：
Nedivi E