Identification of neural circuits involved in female genital responses in the rat: a dual virus and anterograde tracing study.
Identification of neural circuits involved in female genital responses in the rat: a dual virus and anterograde tracing study.
复制标题
鉴定参与大鼠雌性生殖器反应的神经回路：双重病毒和顺行追踪研究。
DOI：
10.1152/ajpregu.00864.2005
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Murphy,AZ
中科院分区：
文献类型：
--
作者：
Marson,L;Murphy,AZ
阴蒂和阴道的脊髓和周围神经支配已相当了解。然而，人们对这些骨盆结构的脊柱上控制知之甚少。多突触示踪剂伪狂犬病病毒（PRV）被用来绘制支配阴蒂和阴道的大脑神经元的图谱。为了描绘 PRV 标记细胞的前脑输入，在病毒注射前 10 天，将顺行示踪剂生物素化葡聚糖胺注射到内侧视前区 (MPO)、下丘脑腹内侧核 (VMN) 或中脑导水管周围灰质 (PAG)。这些大脑区域与女性生殖行为的各个方面密切相关。在阴道和阴蒂注射病毒（4天）后，在室旁核（PVN）、巴林顿核、A5区和巨细胞核（nPGi）中观察到PRV标记的细胞。病毒给药后 5 天，在视前区、VMN、PAG 和外侧下丘脑内观察到额外的 PRV 标记细胞。来自 MPO 的顺行标记主要在下丘脑背侧 PVN、腹外侧 VMN (VMNvl)、尾侧 PAG 和 nPGi 内的 PRV 阳性细胞中终止。来自 VMN 的顺行标记在 MPO 和外侧/腹外侧 PAG 中的 PRV 阳性细胞中终止。 PAG 的顺行标记在 PVN、腹侧下丘脑和 nPGi 的 PRV 阳性细胞中终止。下丘脑外侧、巴林顿核和腹内侧延髓中的突触标记细胞接受所有三个来源的神经支配。这些研究共同确定了参与女性性反应神经控制的几个中枢神经系统（CNS）部位。他们还提供了第一个数据，证明了支配阴蒂和阴道的 MPO、VMNvl 和 PAG 与 CNS 区域之间的联系，为这些区域在女性生殖器反应中发挥重要作用提供了支持。
The spinal and peripheral innervation of the clitoris and vagina are fairly well understood. However, little is known regarding supraspinal control of these pelvic structures. The multisynaptic tracer pseudorabies virus (PRV) was used to map the brain neurons that innervate the clitoris and vagina. To delineate forebrain input on PRV-labeled cells, the anterograde tracer biotinylated dextran amine was injected in the medial preoptic area (MPO), ventromedial nucleus of the hypothalamus (VMN), or the midbrain periaqueductal gray (PAG) 10 days before viral injections. These brain regions have been intimately linked to various aspects of female reproductive behavior. After viral injections (4 days) in the vagina and clitoris, PRV-labeled cells were observed in the paraventricular nucleus (PVN), Barrington’s nucleus, the A5 region, and the nucleus paragigantocellularis (nPGi). At 5 days postviral administration, additional PRV-labeled cells were observed within the preoptic region, VMN, PAG, and lateral hypothalamus. Anterograde labeling from the MPO terminated among PRV-positive cells primarily within the dorsal PVN of the hypothalamus, ventrolateral VMN (VMNvl), caudal PAG, and nPGi. Anterograde labeling from the VMN terminated among PRV-positive cells in the MPO and lateral/ventrolateral PAG. Anterograde labeling from the PAG terminated among PRV-positive cells in the PVN, ventral hypothalamus, and nPGi. Transynaptically labeled cells in the lateral hypothalamus, Barrington’s nucleus, and ventromedial medulla received innervation from all three sources. These studies, together, identify several central nervous system (CNS) sites participating in the neural control of female sexual responses. They also provide the first data demonstrating a link between the MPO, VMNvl, and PAG and CNS regions innervating the clitoris and vagina, providing support that these areas play a major role in female genital responses.