A turbine oil-degrading bacterial consortium from soils of oil fields and its characteristics
A turbine oil-degrading bacterial consortium from soils of oil fields and its characteristics
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DOI：
10.1016/j.ibiod.2007.08.001
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发表时间：
2008-04-01
影响因子：
4.8
通讯作者：
Okuyama, Hidetoshi
中科院分区：
文献类型：
--
作者：
Ito, Hitoshi;Hosokawa, Reia;Okuyama, Hidetoshi
从油田采集的土壤样品中，通过反复富集法获得了一株能够降解汽轮机油的微生物群，其主要成分为难降解的环烷烃和异构烷烃。当这个名为Atsuta A的财团在含有0.5%(w/v)TuO的最低盐介质中培养时，在30℃和pH为7的条件下，它在5天内降解了90%的TuO。虽然从Atstita A联合体中分离到9株细菌，但单独的菌株和它们的混合物对TuO的降解作用可以忽略不计。利用16S rRNA基因的聚合酶链式反应-变性梯度凝胶电泳法(DGGE)研究了陀螺在降解过程中群落结构的变化。在孵化过程中，在至少23条DGGE条带中，有4条主要条带(F、K、N和T)的强度随着时间的推移而显著增加。DGGE谱带F、K和N与以前分离的物种的谱带一致。然而，DGGE条带T并不对应于任何分离株。从T带收集的16S rRNA基因序列与一株属于伽马变形杆菌的不可培养菌株的16S rRNA基因序列同源性为98%。财团中TuO的降解可能通过T带对应的不可培养物种与财团中其他菌株的合作发生，包括F、K和N(C)2007 Elsevier Ltd.。保留所有权利。
A microbial consortium capable of degrading turbine oil (TuO), which consisted mainly of recalcitrant cycloalkanes and isoalkanes, was obtained from a soil sample collected from oil fields using repeated enrichment. When this consortium, named Atsuta A, was cultured in minimal salts medium containing 0.5% (w/v) TuO, it degraded 90% of TuO at 30 degrees C and pH 7 over 5 days. Although nine bacterial strains were isolated from the Atstita A consortium, TuO degradation by the individual isolates and by a mixture of them was negligible. The community structure of the consortium, which was investigated by PCR-denaturing gradient gel electrophoresis (DGGE) targeting 16S rRNA genes, changed significantly during the degradation of TuO. Four major bands (F, K, N and T) out of at least 23 DGGE bands significantly increased in intensity over time during incubation. The DGGE bands F, K and N corresponded to those of previously isolated species. However, DGGE band T did not correspond to any isolated strain. The 16S rRNA gene sequence collected from band T was 98% homologous to that of an unculturable strain belonging to the gamma-Proteobacteria. The degradation of TuO in the consortium may occur by cooperation between the unculturable species corresponding to band T and other strains in the consortium, including species corresponding to bands F, K and N. (C) 2007 Elsevier Ltd. All rights reserved.