ANALYSIS OF THE ROLE OF ASTRAL RAYS IN PRONUCLEAR MIGRATION IN SAND DOLLAR EGGS BY THE COLCEMID-UV METHOD
ANALYSIS OF THE ROLE OF ASTRAL RAYS IN PRONUCLEAR MIGRATION IN SAND DOLLAR EGGS BY THE COLCEMID-UV METHOD
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DOI：
10.1111/j.1440-169x.1986.00143.x
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发表时间：
1986-01-01
影响因子：
2.5
通讯作者：
HIRAMOTO, Y
中科院分区：
文献类型：
--
作者：
HAMAGUCHI, MS;HIRAMOTO, Y
用秋水仙胺紫外分光光度法研究了沙枣卵中精子囊的形成、迁移和受精过程中雌雄原核的迁移。用紫外线（波长约365min）照射秋水仙碱海水中的卵，在含有精子头部的有限区域内形成精子星，精子星在形成过程中向照射区域的中心迁移。当精子星形成后，紫外线照射区域发生位移或形状改变时，精子星迁移到新的紫外线照射区域的中心。精子星的迁移方向与最长星光的方向一致。精子星状体迁移不需要星状体射线与卵表面直接接触。当用紫外线照射含有精子集中区和雌性原核的区域时，雌性原核在它们被星光连接后向精子星的中心迁移。当紫菀和雌原核之间的区域被紫外光遮蔽时，迁移受到抑制。这些结果表明，雌原核是通过与精子星之间的吸引力迁移到精子星上的。
The formation and migration of the sperm aster, and the migration of male and female pronuclei during fertilization were investigated in the eggs of the sand dollar, Clypeaster japonicus using the Colcemid-UV method. When an egg in Colcemid sea water was irradiated locally with UV light (about 365 min wavelength) at a limited region containing sperm head, a sperm aster formed in this region, and migrated to the center of the UV-irradiated region during its formation. When the UV-irradiated region was displaced or its shape was changed after the formation of the sperm aster, the aster migrated to the center of the new UV-irradiated region. The direction of the migration of the sperm aster coincided with the direction of the longest astral rays. Direct contact between astral rays and the egg surface was not essential for sperm aster migration. When a region containing both the sperm concentration and the female pronucleus was irradiated with UV light, the female pronucleus migrated toward the center of the sperm aster after they were connected by astral rays. The migration was suppressed when UV light was shaded over the region between the aster and the female pronucleus. These results suggest that the female pronucleus migrates to the sperm aster by attractive force between them.